Bacterial Endo-Symbiont Inhabiting Durio zibethinus leaves and their Antibacterial Potential

What is it about?

Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytes. In the present study, endophyte was isolated from the leaves of Durio zibethinus. The selected endophyte was identified by 16s rRNA partial genome sequencing and investigated for their antimicrobial activity.

Featured Image

Why is it important?

The preliminary phytochemical test was conducted for the affirmation of phytoconstituents in the endophytic crude extract (DZLM). Antimicrobial activity was assessed against seven human pathogenic ATCC strains. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were recorded. The selected MIC dose was screened by Kirby-Bauer agar well diffusion method. The pre-screening of DZLM showed the presence of various phytoconstituents. DZLM exhibited the highest MIC and MBC of 250 μg/mL and 500 μg/mL respectively, against Bacillus subtilis and Staphylococcus aureus. At MIC of 250 μg/mL, DZLM portrayed significant inhibition zone against ATCC strains comparable to gentamicin.

Perspectives