What is it about?
Mice lacking follicle-stimulating hormone (FSH) are infertile, do not cycle, and do not produce estrogen. Using 3 distinct genotypes of mice- those with intact FSH, no FSH and rescued with FSH, we identified FSH-responsive genes in vivo in ovaries by RNS-Seq. We also mapped how these genes and their regulators are estrous stage specifically expressed in vivo.
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Why is it important?
Contrary to previous in vitro culture studies, we used in vivo genetic approaches to identify FSH-responsive genes in mouse ovaries. We also defined how these genes and their regulators (transcription factors and RNA-binding proteins) behave in distinct estrous stages providing a molecular framework for FSH action in ovaries. Our studies may eventually allow us to identify potential targets for fertility modulation in women.
Perspectives
It was a great pleasure to collaborate with my other Authors at CU Anschutz and learn how to integrate genetics/physiology with bioinformatics. This allowed me to obtain novel insights into the complexities of FSH action in ovaries in vivo in the context of mouse estrous cycles.
T. Rajendra Kumar
University of Colorado
Read the Original
This page is a summary of: Identification of FSH-regulated and estrous stage–specific transcriptional networks in mouse ovaries, Proceedings of the National Academy of Sciences, February 2025, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2411977122.
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