Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract

What is it about?

This study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of Leea indica leaf ethanol extract. Phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, DPPH radical scavenging effect, FeCl3 reducing power, DMSO superoxide scavenging effect and Iron chelating effects were studied by established methods. Antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine shrimp bioassay, respectively. Results showed the total phenolic content 24.00 ± 0.81 g GAE/100 g, total flavonoid content 194.68 ± 2.43 g quercetin/100 g and total antioxidant capacity 106.61 ± 1.84 g AA/100 g dry extract. Significant (P < 0.05) IC50 values compared to respective standards were recorded in DPPH radical scavenging (139.83 ± 1.40 microgm/ml), FeCl3 reduction (16.48 ± 0.64 microgm/ml), DMSO superoxide scavenging (676.08 ± 5.80 microgm/ml) and Iron chelating (519.33 ± 16.96 microgm/ml) methods. In antibacterial screening, the extract showed significant (P < 0.05) zone of inhibitions compared to positive controls Ampicillin and Tetracycline against Gram-positive Bacillus subtilis, Bacillus cereus, Bacillus megaterium, and Staphylococcus aureus and Gram-negative Salmonella typhi, Salmonella paratyphi, Pseudomonas aeroginosa, Shigella dysenteriae, Vibrio cholerae, and Escherichia coli. Significant minimum inhibitory concentrations compared to tetracycline were obtained against the above organisms. In antifungal assay, the extract inhibited the growth of Aspergillus flavus, Candida albicans and Fusarium equisetii by 38.09 ± 0.59, 22.58 ± 2.22, and 22.58 ± 2.22%, respectively. The extract showed a significant LC50 value compared to vincristine sulfate in cytotoxic assay. The results evidenced the potential antioxidative, antimicrobial and cytotoxic capacities of Leea indica leaf extract to be processed for pharmaceutical use.

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Why is it important?

In this study, we reported the further progress whereby the ethanol extract of L. indica was subjected to an analysis of phytochemical status, total phenolic content, total flavonoid, total antioxidant capacity, DPPH radical scavenging effect, FeCl3 reducing effect, DMSO superoxide scavenging effect and Iron chelating effect. This study also reported the antibacterial, antifungal, and cytotoxic activities of the leaf extract using reference standards in each case.

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