What is it about?
Instantaneous removal of proteins that regulate microtubule assembly produces a distinctly different, even opposite, effect on microtubules than the more commonly employed methods of slow overnight protein depletion.
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Why is it important?
This study shows that the response of tubulin autoregulation contributes to the phenotype recorded from slow depletion of microtubule regulators and illustrates the importance of considering the time course of experimental manipulations in cells.
Perspectives
Rapid removal or relocation of individual cellular regulators can tell us what the instantaneous activity of a protein is in the cell in the context of all the other contributing proteins and without the added complication of compensatory responses.
Linda Wordeman
University of Washington
Read the Original
This page is a summary of: Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins, Molecular Biology of the Cell, February 2016, American Society for Cell Biology (ASCB),
DOI: 10.1091/mbc.e15-11-0803.
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