Making SEA PS resin, SEA peptides and thioesters

What is it about?

Native Chemical Ligation and SEA native peptide ligation make use of peptide thioesters and SEA peptides. This article describes in full detail the method to produce SEA resin, then SEA peptides using standard solid phase peptide synthesis and how to convert SEA peptides into peptide thioesters. The preparation of these useful peptide segments is made easy and can be implemented in all labs equiped with Fmoc SPPS peptide synthesizers.

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Why is it important?

SEA ligation is a useful complement to Native Chemical Ligation. This paper gives all the details to master SEA peptide synthesis, and how to use SEA peptides as starting material to produce peptide thioesters using a mild and robust protocol. This paper resumes the observations and studies made over five years since SEA ligation was introduced by us, and provides all the information to exploit the powerfulness of SEA chemistry.