All Stories

  1. High-pressure protein crystal structure analysis of Escherichia coli dihydrofolate reductase complexed with folate and NADP+

    Article • Acta Crystallographica Section D Structural Biology, September 2018, International Union of Crystallography

    Professor Nobuhisa Watanabe

  2. Beamline on the 5T superbend. High-pressure protein crystallography with DAC can also be performed.

    Article • Journal of Synchrotron Radiation, January 2017, International Union of Crystallography

    Professor Nobuhisa Watanabe

  3. Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution

    Article • Extremophiles, February 2016, Springer Science + Business Media

    Professor Nobuhisa Watanabe

  4. Unique Flap Conformation in an HIV-1 Protease with High-Level Darunavir Resistance

    Article • Frontiers in Microbiology, February 2016, Frontiers

    Professor Nobuhisa Watanabe, Dr Yasumasa Iwatani

  5. Vif-APOBEC3F Interaction Interfaces

    Article • Journal of Virology, November 2015, ASM Journals

    Dr Yasumasa Iwatani, Professor Nobuhisa Watanabe

  6. How the high pKa of Glu35 of HEWL is realized

    Article • Acta Crystallographica Section D Biological Crystallography, March 2015, International Union of Crystallography

    Professor Nobuhisa Watanabe

  7. Definition of the transcription factor TdIF1 consensus-binding sequence through genomewide mapping of its binding sites

    Article • Genes to Cells, January 2015, Wiley

    Professor Nobuhisa Watanabe

  8. two-ion mechanism of HindIII was observed

    Article • Acta Crystallographica Section D Biological Crystallography, January 2015, International Union of Crystallography

    Professor Nobuhisa Watanabe

  9. High Pressure Macromolecular Crystallography

    Article • January 2015, Springer Science + Business Media

    Professor Nobuhisa Watanabe

  10. Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomyces coelicolor A3(2)

    Article • FEBS Letters, October 2014, Wiley

    Professor Nobuhisa Watanabe, Ui Okada

  11. Structural study of the pressure adaptation of proteins from deep-sea bacteria

    Article • Acta Crystallographica Section A Foundations and Advances, August 2014, International Union of Crystallography

    Professor Nobuhisa Watanabe

  12. SAD experiment using the wavelength of 2.7 Å or longer

    Article • Acta Crystallographica Section A Foundations and Advances, August 2014, International Union of Crystallography

    Professor Nobuhisa Watanabe

  13. Crystal structure of the Vif-interaction domain of the anti-viral APOBE3F

    Article • Acta Crystallographica Section A Foundations and Advances, August 2014, International Union of Crystallography

    Professor Nobuhisa Watanabe

  14. Pressure effects on the chimeric 3-isopropylmalate dehydrogenases of the deep-sea piezophilicShewanella benthicaand the atmospheric pressure-adaptedShewanella oneidensis

    Article • Bioscience Biotechnology and Biochemistry, March 2014, Taylor & Francis

    Professor Nobuhisa Watanabe

  15. New methodologies at PF AR-NW12A: the implementation of high-pressure macromolecular crystallography

    Article • Journal of Synchrotron Radiation, October 2013, International Union of Crystallography

    Professor Nobuhisa Watanabe

  16. SCO4008, a Putative TetR Transcriptional Repressor from Streptomyces coelicolor A3(2), Regulates Transcription of sco4007 by Multidrug Recognition

    Article • Journal of Molecular Biology, September 2013, Elsevier

    Professor Nobuhisa Watanabe, Ui Okada

  17. TdIF1 Recognizes a Specific DNA Sequence through Its Helix-Turn-Helix and AT-Hook Motifs to Regulate Gene Transcription

    Article • PLoS ONE, July 2013, PLOS

    Professor Nobuhisa Watanabe

  18. Structural and genomic DNA analysis of a putative transcription factor SCO5550 from Streptomyces coelicolor A3(2): Regulating the expression of gene sco5551 as a transcriptional activator with a novel dimer shape

    Article • Biochemical and Biophysical Research Communications, May 2013, Elsevier

    Professor Nobuhisa Watanabe

  19. Crystal sample pins and a storage cassette system compatible with the protein crystallography beamlines at both the Photon Factory and SPring-8

    Article • Journal of Applied Crystallography, October 2012, International Union of Crystallography

    Professor Nobuhisa Watanabe

  20. The APOBEC3C crystal structure and the interface for HIV-1 Vif binding

    Article • Nature Structural & Molecular Biology, September 2012, Nature

    Professor Nobuhisa Watanabe, Dr Yasumasa Iwatani

  21. Mechanistic Insights into the 1,3-Xylanases: Useful Enzymes for Manipulation of Algal Biomass

    Article • Journal of the American Chemical Society, February 2012, American Chemical Society (ACS)

    Professor Nobuhisa Watanabe

  22. Structural analysis of 3-isopropylmalate dehydrogenase from the obligate piezophileShewanella benthicaDB21MT-2 and the nonpiezophileShewanella oneidensisMR-1

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, February 2012, International Union of Crystallography

    Professor Nobuhisa Watanabe

  23. High-pressure-induced water penetration into 3-isopropylmalate dehydrogenase

    Article • Acta Crystallographica Section D Biological Crystallography, February 2012, International Union of Crystallography

    Professor Nobuhisa Watanabe

  24. The Structure of Physarum polycephalum Hemagglutinin I Suggests a Minimal Carbohydrate Recognition Domain of Legume Lectin Fold

    Article • Journal of Molecular Biology, January 2011, Elsevier

    Professor Nobuhisa Watanabe

  25. Crystal Structures of the Multidrug Binding Repressor Corynebacterium glutamicum CgmR in Complex with Inducers and with an Operator

    Article • Journal of Molecular Biology, October 2010, Elsevier

    Professor Nobuhisa Watanabe

  26. Semi-automated protein crystal mounting device for the sulfur single-wavelength anomalous diffraction method

    Article • Journal of Applied Crystallography, February 2010, International Union of Crystallography

    Professor Nobuhisa Watanabe

  27. Fully Automated Data Collection Using PAM and the Development of PAM∕SPACE Reversible Cassettes

    Article • January 2010, American Institute of Physics

    Professor Nobuhisa Watanabe

  28. Structure of putative 4-amino-4-deoxychorismate lyase fromThermus thermophilusHB8

    Article • Acta Crystallographica Section F Structural Biology and Crystallization Communications, November 2009, International Union of Crystallography

    Professor Nobuhisa Watanabe, Dr Svetlana Antonyuk

  29. Structures of restriction endonucleaseHindIII in complex with its cognate DNA and divalent cations

    Article • Acta Crystallographica Section D Biological Crystallography, November 2009, International Union of Crystallography

    Professor Nobuhisa Watanabe

  30. X-ray crystallography and structural stability of digestive lysozyme from cow stomach

    Article • FEBS Journal, April 2009, Wiley

    Professor Nobuhisa Watanabe

  31. Crystallographic Structure And Structural Stability Of Vertebrate Digestive Lysozyme

    Article • Biophysical Journal, February 2009, Elsevier

    Professor Nobuhisa Watanabe

  32. Structure of mannosyl-3-phosphoglycerate phosphatase fromPyrococcus horikoshii

    Article • Acta Crystallographica Section D Biological Crystallography, November 2008, International Union of Crystallography

    Professor Nobuhisa Watanabe

  33. A water molecule identified as a substrate of enzymatic hydrolysis of cellulose: A statistical-mechanics study

    Article • Chemical Physics Letters, November 2008, Elsevier

    Professor Nobuhisa Watanabe

  34. Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)

    Article • Protein and Peptide Letters, July 2008, Bentham Science Publishers

    Professor Nobuhisa Watanabe

  35. A Helical String of Alternately Connected Three-Helix Bundles for the Cell Wall-Associated Adhesion Protein Ebh from Staphylococcus aureus

    Article • Structure, March 2008, Elsevier

    Professor Nobuhisa Watanabe

  36. Spontaneous asparaginyl deamidation of canine milk lysozyme under mild conditions

    Article • Proteins Structure Function and Bioinformatics, January 2008, Wiley

    Professor Nobuhisa Watanabe

  37. Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 fromStreptomyces coelicolor

    Article • Acta Crystallographica Section D Biological Crystallography, January 2008, International Union of Crystallography

    Professor Nobuhisa Watanabe, Ui Okada

  38. SAD phasing by OASIS at different resolutions down to 0.30 nm and below

    Article • Chinese Physics B, January 2008, Institute of Physics Publishing

    Professor Nobuhisa Watanabe

  39. Crystal structure of the PH1932 protein, a unique archaeal ArsR type winged-HTH transcription factor from Pyrococcus horikoshii OT3

    Article • Proteins Structure Function and Bioinformatics, December 2007, Wiley

    Professor Nobuhisa Watanabe

  40. Crystallization and Preliminary X-Ray Studies of the Unliganded Wild-Type Bovine Thrombin

    Article • Protein and Peptide Letters, September 2007, Bentham Science Publishers

    Professor Nobuhisa Watanabe

  41. Crystal structure analysis reveals a novel forkhead-associated domain of ESAT-6 secretion system C protein in Staphylococcus aureus

    Article • Proteins Structure Function and Bioinformatics, August 2007, Wiley

    Professor Nobuhisa Watanabe

  42. Identification of Multiple Amyloidogenic Sequences in Laminin-1 †

    Article • Biochemistry, April 2007, American Chemical Society (ACS)

    Professor Nobuhisa Watanabe

  43. タンパク質結晶構造解析における長波長X線利用

    Article • Seibutsu Butsuri, January 2007, Biophysical Society of Japan

    Professor Nobuhisa Watanabe

  44. DNA Recognition Mechanism of the ONECUT Homeodomain of Transcription Factor HNF-6

    Article • Structure, January 2007, Elsevier

    Professor Nobuhisa Watanabe

  45. From phasing to structure refinement in-house: Cr/Cu dual-wavelength system and a loopless free crystal-mounting method

    Article • Acta Crystallographica Section D Biological Crystallography, July 2006, International Union of Crystallography

    Professor Nobuhisa Watanabe

  46. SAD phasing byOASIS-2004: case studies of dual-space fragment extension

    Article • Acta Crystallographica Section D Biological Crystallography, July 2006, International Union of Crystallography

    Professor Nobuhisa Watanabe

  47. Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA

    Article • Protein and Peptide Letters, May 2006, Bentham Science Publishers

    Professor Nobuhisa Watanabe

  48. Structure of the catalytic nucleotide-binding subunit A of A-type ATP synthase fromPyrococcus horikoshiireveals a novel domain related to the peripheral stalk

    Article • Acta Crystallographica Section D Biological Crystallography, April 2006, International Union of Crystallography

    Professor Nobuhisa Watanabe

  49. Structural analysis of the transcriptional regulator homolog protein from Pyrococcus horikoshii OT3

    Article • Proteins Structure Function and Bioinformatics, February 2006, Wiley

    Professor Nobuhisa Watanabe, Ui Okada

  50. Development of the Loopless Free Crystal Mounting Method and Crystal Structure Determination Using Cr K.ALPHA. X-rays Without Any Modifications Nor Derivatizations.

    Article • Nihon Kessho Gakkaishi, January 2006, The Crystallographic Society of Japan

    Professor Nobuhisa Watanabe

  51. Comparison of phasing methods for sulfur-SAD using in-house chromium radiation: case studies for standard proteins and a 69 kDa protein

    Article • Acta Crystallographica Section D Biological Crystallography, October 2005, International Union of Crystallography

    Professor Nobuhisa Watanabe

  52. Structure determination of a novel protein by sulfur SAD using chromium radiation in combination with a new crystal-mounting method

    Article • Acta Crystallographica Section D Biological Crystallography, July 2005, International Union of Crystallography

    Professor Nobuhisa Watanabe

  53. Modified Nextal crystallization device forin situinspection of protein crystal quality

    Article • Journal of Applied Crystallography, March 2005, International Union of Crystallography

    Professor Nobuhisa Watanabe

  54. Crystal structure of PH0010 from Pyrococcus horikoshii, which is highly homologous to human AMMECR 1C-terminal region

    Article • Proteins Structure Function and Bioinformatics, November 2004, Wiley

    Professor Nobuhisa Watanabe

  55. Structural and Enzymatic Properties of 1-Aminocyclopropane-1-carboxylate Deaminase Homologue from Pyrococcus horikoshii

    Article • Journal of Molecular Biology, August 2004, Elsevier

    Professor Nobuhisa Watanabe

  56. Crystal structure of the ATP-binding cassette of multisugar transporter from Pyrococcus horikoshii OT3

    Article • Proteins Structure Function and Bioinformatics, July 2004, Wiley

    Professor Nobuhisa Watanabe

  57. Preparation and preliminary X-ray analysis of the catalytic module of β-1,3-xylanase from the marine bacteriumVibriosp. AX-4

    Article • Acta Crystallographica Section D Biological Crystallography, July 2004, International Union of Crystallography

    Professor Nobuhisa Watanabe

  58. Crystallization and preliminary X-ray diffraction study of the catalytic subunit of archaeal H+-transporting ATP synthase fromPyrococcus horikoshiiOT3

    Article • Acta Crystallographica Section D Biological Crystallography, July 2004, International Union of Crystallography

    Professor Nobuhisa Watanabe

  59. Structure analysis of PH1161 protein, a transcriptional activator TenA homologue from the hyperthermophilic archaeonPyrococcus horikoshii

    Article • Acta Crystallographica Section D Biological Crystallography, May 2004, International Union of Crystallography

    Professor Nobuhisa Watanabe

  60. Crystal structure of conserved protein PH1136 from Pyrococcus horikoshii

    Article • Proteins Structure Function and Bioinformatics, February 2004, Wiley

    Professor Nobuhisa Watanabe

  61. Crystal structure of human p120 homologue protein PH1374 from Pyrococcus horikoshii

    Article • Proteins Structure Function and Bioinformatics, January 2004, Wiley

    Professor Nobuhisa Watanabe

  62. Crystal structure of hypothetical protein PH0642 fromPyrococcus horikoshii at 1.6Å resolution

    Article • Proteins Structure Function and Bioinformatics, January 2004, Wiley

    Professor Nobuhisa Watanabe

  63. Crystal structure of hypothetical protein PH0828 fromPyrococcus horikoshii

    Article • Proteins Structure Function and Bioinformatics, January 2004, Wiley

    Professor Nobuhisa Watanabe

  64. Ile-Lys-Val-Ala-Val (IKVAV)-containing laminin α1 chain peptides form amyloid-like fibrils

    Article • FEBS Letters, September 2002, Wiley

    Professor Nobuhisa Watanabe

  65. Semi-automatic protein crystallization system that allows in situ observation of X-ray diffraction from crystals in the drop

    Article • Acta Crystallographica Section D Biological Crystallography, September 2002, International Union of Crystallography

    Professor Nobuhisa Watanabe

  66. The crystal structure of human MRP14 (S100A9), a Ca2+-dependent regulator protein in inflammatory process

    Article • Journal of Molecular Biology, February 2002, Elsevier

    Professor Nobuhisa Watanabe

  67. The Three-Dimensional Structure of Septum Site-Determining Protein MinD from Pyrococcus horikoshii OT3 in Complex with Mg-ADP

    Article • Structure, September 2001, Elsevier

    Professor Nobuhisa Watanabe, Professor Masaru Tanokura

  68. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the human calcium-binding protein MRP14 (S100A9)

    Article • Acta Crystallographica Section D Biological Crystallography, July 2001, International Union of Crystallography

    Professor Nobuhisa Watanabe

  69. Automatic Weissenberg data collection system for time-resolved protein crystallography

    Article • Nuclear Instruments and Methods in Physics Research Section A Accelerators Spectrometers Detectors and Associated Equipment, July 2001, Elsevier

    Professor Nobuhisa Watanabe

  70. The MinD protein from the hyperthermophilic archaeonPyrococcus horikoshii: crystallization and preliminary X-ray analysis

    Article • Acta Crystallographica Section D Biological Crystallography, May 2001, International Union of Crystallography

    Professor Nobuhisa Watanabe

  71. A very simple and cheap greasing device for crystallization plates

    Article • Journal of Applied Crystallography, April 2001, International Union of Crystallography

    Professor Nobuhisa Watanabe

  72. Focusing Monochromator for Protein Crystallography.

    Article • Nihon Kessho Gakkaishi, January 2000, The Crystallographic Society of Japan

    Professor Nobuhisa Watanabe

  73. Rotated-inclined focusing monochromator with simultaneous tuning of asymmetry factor and radius of curvature over a wide wavelength range

    Article • Journal of Synchrotron Radiation, March 1999, International Union of Crystallography

    Professor Nobuhisa Watanabe

  74. Protein Crystallography and Structural Biology. Recent Progress of Methodology in Protein Crystallography. Using Synchrotron Radiation in Protein Crystallography.

    Article • Nihon Kessho Gakkaishi, January 1996, The Crystallographic Society of Japan

    Professor Nobuhisa Watanabe

  75. Macromolecular crystallography station BL‐18B at the Photon Factory

    Article • Review of Scientific Instruments, February 1995, American Institute of Physics

    Professor Nobuhisa Watanabe

  76. ＳＰｒｉｎｇ‐８と生物物理学 第２世代から第３世代放射光に向けて タンパク質Ｘ線結晶解析 時間分割タンパク質Ｘ線結晶構造解析 ピコ秒の時間分解能を目指して

    Article • Seibutsu Butsuri, January 1995, Biophysical Society of Japan

    Professor Nobuhisa Watanabe

  77. 特集 タンパク質結晶学の新世代 新しいターゲットへの挑戦 時間分割ラウエ法と動的構造解析 分子反応は見えるか？

    Article • Seibutsu Butsuri, January 1993, Biophysical Society of Japan

    Professor Nobuhisa Watanabe

  78. Application of transform theory to resonance cars excitation profiles of a cyanine dye

    Article • Journal of Raman Spectroscopy, September 1987, Wiley

    Professor Nobuhisa Watanabe