Synthetic seed of Bacopa monnieri

What is it about?

The present study reports on the influence of sodium alginate and calcium chloride (CaCl2.H20) levels on proficient synthetic seed (synseed) formation of Bacopa monnieri (L.) Pennell. In vitro nodal segments (~4 mm) were employed for encapsulation wherein aliquots of 1.5-4% (w/v) (i.e. 1.5, 2, 2.5, 3, 3.5, and 4%) sodium alginate containing individual nodal segments were dropped in either 75 mM or 100 mM calcium chloride. Uniform spherical beads were obtained using 2.5% sodium alginate and 75 mM calcium chloride. Earliest conversion (into plantlet with shoot and root) of synseeds derived from optimized sodium alginate and calcium chloride level, was achieved on half-strength Murashige and Skoog semisolid regrowth medium. The synseeds displayed distinctive characters in their response to variable levels of sodium alginate and calcium chloride. The comparatively lower (1.5 and 2%) or higher (3, 3.5 and 4%) sodium alginate levels accompanied with more concentrated (100 mM) calcium chloride resulted either in squashy, fragile or harder and tail-forming beads, respectively.

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