Detection of Campylobacter jejuni from fresh produce: comparison of culture- and PCR-based techniques, and metagenomic approach for analyses of the microbiome before and after enrichment

What is it about?

 We compared the efficiency of culture-based methods with or without membrane filtration, real-time PCR and digital droplet PCR (ddPCR) for the detection of Campylobacter in fresh produce. _x000D_  There was no significant difference in the detection efficiency of positive Campylobacter isolates from coleslaw and lettuce salad using four detection methods. _x000D_  However, for sprout samples, the detection efficiency of the culture method was significantly lower than those of the two PCR assays and the filtration method. _x000D_

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