What is it about?
By inoculating 30 Campylobacter spp. strains and 25 ESBL-producing E. coli strains onto Karmali agar and T-Karmali agar containing various concentrations of the antibacterial agent, we determined the optimum concentration of tazobactam to be 4 mg/liter.
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Why is it important?
The Campylobacter spp. isolation rate on T-Karmali agar (13.3%) was higher than that on Karmali agar (8.3%), although the difference was not significant (P > 0.05). However, T-Karmali agar showed a significantly greater selectivity than Karmali agar, as evaluated by comparing the numbers of contaminated agar plates (20.8 versus 82.5%; P < 0.05) and the growth indexes (1.36 versus 2.83) of competing flora. The predominant competing flora on Karmali and T-Karmali agar were identified as ESBL-producing E. coli .
Perspectives
T-Karmali agar might be effective for determining the real prevalence of Campylobacter in raw poultry and, especially, contamination with ESBL-producing E. coli .
Professor Kun-Ho Seo
Konkuk University
Read the Original
This page is a summary of: Improvement of Karmali Agar by Supplementation with Tazobactam for Detecting Campylobacter in Raw Poultry, Journal of Food Protection, November 2016, International Association for Food Protection,
DOI: 10.4315/0362-028x.jfp-16-105.
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