Fourier Transform Infrared and Ultraviolet-Visible Spectroscopic Characterization of Ammonium Acetate and Ammonium Chloride: An Impact of Biofield Treatment

What is it about?

Ammonium acetate and ammonium chloride are the white crystalline solid inorganic compounds having wide application in synthesis and analytical chemistry. The aim of present study was to evaluate the impact of biofield treatment on spectral properties of inorganic salt like ammonium acetate and ammonium chloride. The study was performed in two groups of each compound i.e., control and treatment. Treatment groups were received Mr. Trivedi’s biofield treatment. Subsequently, control and treated groups were evaluated using Fourier Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopy. FT-IR spectrum of treated ammonium acetate showed the shifting in wavenumber of vibrational peaks with respect to control. Like, the N-H stretching was shifted from 3024-3586 cm-1 to 3033-3606 cm-1, C-H stretching from 2826-2893 cm-1 to 2817-2881 cm-1, C=O asymmetrical stretching from 1660-1702 cm-1 to 1680-1714 cm-1, N-H bending from 1533-1563 cm-1 to 1506-1556 cm-1 etc. Treated ammonium chloride showed the shifting in IR frequency of three distinct oscillation modes in NH4 ion i.e., at ν1, 3010 cm-1 to 3029 cm-1; ν2, 1724 cm-1 to 1741 cm-1; and ν3, 3156 cm-1 to 3124 cm-1. The N-Cl stretching was also shifted to downstream region i.e., from 710 cm-1 to 665 cm-1 in treated ammonium chloride. UV spectrum of treated ammonium acetate showed the absorbance maxima (λmax) at 258.0 nm that was shifted to 221.4 nm in treated sample. UV spectrum of control ammonium chloride exhibited two absorbance maxima (λmax) i.e., at 234.6 and 292.6 nm, which were shifted to 224.1 and 302.8 nm, respectively in treated sample. Overall, FT-IR and UV data of both compounds suggest an impact of biofield treatment on atomic level i.e., at force constant, bond strength, dipole moments and electron transition energy between two orbitals of treated compounds as compared to respective control.

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Why is it important?

Ammonium acetate (CH3COONH4) is a white crystalline solid, water soluble compound derived from the chemical reaction ammonia and acetic acid. Being a salt of weak base and weak acid, it possesses several distinct applications like, it is used as an aqueous buffer for High-Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) and Electrospray Ionization Mass Spectrometry (ESI-MS) of proteins [1,2]. It is also used as a food additive to regulate the acidity. Therapeutically, it is reported as an antidiuretic and antipyretic and also as a nutrient [1,3]. Ammonium acetate is also used as an intermediate and catalyst in numerous chemical reactions [1,4]. On the contrary, ammonium acetate also associated with its toxicities like flaccidity of facial muscles, generalized discomfort, tremor, anxiety, and impairment of motor performance [3]. Ammonium chloride (NH4Cl) is also a white crystalline inorganic salt, having high solubility in water. The natural and mineralogical form of ammonium chloride is known as sal ammoniac. The ammonium chloride has wide application in the field of medicine, agriculture and in food. In medicine, it is used as an expectorant in cough syrup due to irritative effect on the bronchial mucosa. Ammonium chloride causes the nausea and vomiting effects owing to irritative effect on gastric mucosa [5]. It is also used as a systemic acidifying agent for the treatment of severe metabolic alkalosis, and to maintain the urine at acidic pH in the treatment of urinary-tract disorders [6]. In food products, ammonium chloride is used as an additive or feed supplement for cattle and as a nutrient for yeast and other microbes [7,8]. It is also used to improve the crispness of cookies and snacks items. In agriculture, the ammonium chloride is used as an important source of nitrogen in fertilizers [9]. The chemical and physical stability of any chemical compound are most desired qualities that determine its shelf life and effectiveness [10]. Hence, it is advantageous to find out an alternate approach, which could enhance the stability of compounds by altering the structural properties of these compounds. Recently, biofield treatment is reported to alter the physical, and structural properties of various living and non-living substances [11,12]. The relation between mass-energy was described by Einstein through a well-known equation E=mc2 [13]. Planck M gave a hypothesis that energy is a property of matter or substances that neither can be created nor destroyed but can be transmitted to other substances by changing into different forms [14]. According to Maxwell JC, every dynamic process in the human body had an electrical significance [15]. Researchers have experimentally demonstrated the presence of electromagnetic field around the human body using medical technologies such as electromyography, electrocardiography and electroencephalogram [16]. This electromagnetic field of the human body is known as biofield and energy associated with this field is known as biofield energy [17]. Mr. Trivedi has the ability to harness the energy from environment or universe and can transmit into any object (living or nonliving) around this Globe. The object(s) always receive the energy and responding into useful way, this process is known as biofield treatment [11,12]. Mr. Trivedi’s unique biofield treatment is also called as The Trivedi Effect®, and known to alter the characteristics of many things in the verities of research fields including microbiology [11,18], agriculture [19,20], and biotechnology [21,22]. Recently, impact of biofield treatment on atomic, crystalline and powder characteristics as well as spectroscopic characters of different materials were studied and alteration in physical, thermal and chemical properties were reported [12,23,24]. Considering the effects of biofield treatment on various living and nonliving things, the study was aimed to evaluate the impact of biofield treatment on spectral properties of ammonium acetate and ammonium chloride. The effects were analyzed using Fourier Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopic techniques.

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