The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy

What is it about?

Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non-GBM control and non-GBM treatment. The GBM and non-GBM treatment groups were given Mr. Trivedi’s biofield treatment for the assessment of its potential. Two time-lapse (10 hours prior; 10 hours after) video microscopy experiment was performed on tumor and non-tumor brain cells in six replicate (n=6). For each microscopic field, the total cell number was counted and each cell was tracked over the 20 hours period. The potential impact of biofield treatment was assessed by comparing cell death rate in both GBM and non-GBM cells before and after biofield treatment. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. The treated non-GBM cultured cells showed a significant reduction (64%) of cell death rate i.e. protective effects as compared to non-GBM control. Conclusion: Altogether, data suggests that biofield treatment has significantly increased the cell death rate of treated GBM cells and simultaneously boost the viability of normal brain cells. Therefore, biofield treatment could be a suitable alternate treatment strategy for cancer patients in near future.

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Why is it important?

Brain tumors are typically very heterogeneous, aggressive neoplasms at the cellular level which affects both children and adults [1]. Statistics based on cancer research institute explore that 1 in 161 individuals born today with brain cancer at different point of life. In the U.S., 22,850 men and women are diagnosed with brain cancer every year, and 15,320 deaths are caused by this disease [2]. As per World health organization classification, glioblastoma (GBM) is also known as grade IV astrocytoma [3]. GBM is one of the most malignant form of human brain tumor. The mean survival time for GBM patients is approximately 12 months [4]. Time-lapse video microscopy is a technique that aids to assess cellular behavior in real time. This technique is used to collect a two dimensional image data at different time intervals. Then, these data are converted to make a movie. This unique technique is used in various fields of cancer and stem cell biology for assessment of cell translation, division, and death [5,6]. Presently, several strategies are available for the treatment of GBM such as surgical techniques, radiotherapy, chemotherapy and electrochemotherapy [7]. Apart from commonly used chemotherapeutic agents (such as nitrosoureas derivatives, platinum based drugs, and taxol) and gene therapy, as a novel therapeutic modality frequently being used in cancer [8]. However, chemotherapy suffers several major drawbacks such as all tumors are not responsive to chemotherapeutic agents to some extent, incidence of bone marrow cells damage, amenorrhea, alopecia, sexual dysfunction and adversely affect the quality of life of patients [9]. Malignant brain tumors like GBM are very difficult to treat now a days. The difficulties to treat against GBM are due to lack of proper preventive strategies, and unavailability of practical method for screening [10]. Therefore, new, more effective and better tolerated anti-tumor drugs or some alternative treatment strategy are needed. Based on the above lacunas an alternative way which may be useful to kill or eradicates the proliferative tumorous cells and simultaneously defense the normal brain glial cells. Biofield treatment is an alternative approach which may be useful to improve these unfilled spaces associated with cancer patients. The human biofield is the energetic matrix that surrounds the human [11]. It directly links with the cellular activity that allows the DNA to communicate faster than light and maintain intelligence in the organisms [12]. According to universal principles of Maxwell’s equations and principle of reciprocity defines electromagnetic connections related to human biofield [13]. Thus, a human has ability to harness the energy from environment/Universe and can transmit into any object (living or non-living) around the Globe. The object(s) always receive the energy and responded into useful way that is called biofield energy. This process is known as biofield treatment. The biofield can be monitored using electromyography, electrocardiography and electroencephalogram [14]. Mr. Mahendra Trivedi’s unique biofield treatment (The Trivedi Effect) has been well known for altered characteristics features of microbes [15-17], improved the overall productivity of crops [18,19], and also transform the structural, physical and chemical properties of materials [20-23]. Exposure to biofield energy caused an increase in medicinal property, growth, and anatomical characteristics of ashwagandha [24]. On the basis of above facts and literature, present study was undertaken to evaluate the impact of Mr. Trivedi’s biofield treatment on human GBM brain tumor cells.

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