Investigation of Biofield Treatment on Antimicrobial Susceptibility, Biochemical Reaction Pattern and Biotyping of Enteropathogenic Multidrug-Resistant Escherichia coli Isolates

What is it about?

Study background: Multidrug resistant Escherichia coli (MDR E. coli) has become a major health concern, and failure of treatment leads to huge health burden. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on E. coli. Methods: Four MDR clinical lab isolates (LSs) of E. coli (LS 8, LS 9, LS 10, and LS 11) were taken and divided into two groups i.e. control and biofield treated. Control and treated samples were identified with respect to its antimicrobial sensitivity assay, biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with its respective control group. Results: Antimicrobial sensitivity assay showed 50% alteration in sensitivity of total tested antimicrobials in treated group of MDR E. coli isolates. MIC results showed the alteration in MIC of about 40.63% antimicrobials out of thirty two tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Ticarcillin/k-clavulanate showed improved sensitivity (R → I) with decreased MIC value in LS 9 as compared to control. A fourfold and twofold decreased in MIC values were reported in case of piperacillin/tazobactam (in LS 9) and chloramphenicol (in LS 8), respectively as compared to respective control. Biochemical study showed a 39.39% alteration in biochemical reactions after treatment among four isolates of E. coli as compared to control. A significant change in biotype numbers were reported in three clinical isolates (i.e. LS 8, LS 9, and LS 11) of MDR E. coli as compared to control. On the basis of changed biotype number (7774 5272) after biofield treatment, organism with maximum probability was identified as Enterobacter aerogenes in LS 8 as compared to control, (E. coli, 7711 5012). Conclusion: Overall results suggest that Mr Trivedi’s biofield treatment has a significant effect on altering the antimicrobial sensitivity, biochemical reactions and biotype number of MDR isolates of E. coli.

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Why is it important?

Escherichia coli (E. coli) is a Gram-negative, rod shape, and facultative anaerobic bacteria predominantly found in human colonic flora. Despite the fact about E. coli, its commensal nature and common existence in microflora of animal intestine including man, all the E. coli strains are not harmful; sometimes it causes fatal enteric infections in humans as well as mammals and birds [1]. Pathogenic strains of E. coli cause common intestine infection i.e. diarrhea, extra intestinal infections in humans and animals includes urinary tract infections (UTI), meningitis, and septicemia [2]. Apart from these diseases, pathogenic E. coli may be responsible for causing cystitis and pylonephritis, a major cause in approximately 80% of 130-175 million human UTIs [3]. Furthermore, E. coli causing extra intestinal infections are the major Gram-negative bacterial pathogens responsible for neonatal meningitis, and ranks second in an overall cause of the disease after group B streptococcus infections [4,5]. During last few years, increasing emergence and wide dissemination of E. coli isolates show resistance to broad-spectrum antimicrobial agents [6]. MDR isolates are the basic cause of failure in treatment modalities, resulting high rate of morbidity and mortality [7]. An emergence of resistance against multiple antimicrobials drugs is a serious threat to public health, and sometimes no available antimicrobials will be effective to treat the infections caused by MDR E. coli [6,8]. Due to dramatically increase in drug resistance against antibiotics, some alternate approach is required to later the sensitivity pattern of antimicrobials. Recently, biofield treatment on pathogenic microorganism is available as an alternative approach to altering the sensitivity pattern of various antimicrobials [9,10]. Biofield as an energy medicine has been included in complementary and alternative medicine (CAM) therapies, and very commonly practiced in US by professional healthcare representative [11]. CAM therapies are very helpful to improve the human wellbeing and health without having any side effects. Bio-electrographic method is noninvasive technology used to measure the human biofield which can evaluate the physical and emotional heath [12]. The energy exists in various forms that can be produced from different sources such as potential, electrical, kinetic, magnetic, and nuclear energy. The cumulative effect of bio-magnetic and electric field that surrounds the human body is defined as biofield, and the extent of energy associated with biofield is termed as biofield energy. It can be monitored using techniques such as electromyography (EMG), electrocardiography (ECG) and electroencephalogram (EEG) [13]. Mr Mahendra Kumar Trivedi has the ability to harness the energy from environment or universe and can transmit into any living or non-living object(s) around the Universe. The objects always receive the energy and responding into useful way via biofield energy and the process is known as biofield treatment. Mr Trivedi’s unique biofield treatment is also known as The Trivedi Effect® and it was widely studied in the field of material science [14-16], agricultural science [17-19], and in biotechnology [20]. In microbiology, biofield treatment on pathogenic microbes and MDR isolates had been reported to alter the antimicrobial sensitivity, biochemical reactions and biotype number [10,21,22]. In continuation of outstanding results of biofield treatment and clinical significance of MDR E. coli, present work was designed to evaluate the influence of biofield treatment on MDR isolates of E. coli with respect to its antimicrobials susceptibility, biochemical reactions pattern, and biotyping.

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