What is it about?
The amnis flow cytometry captures images of each cell analysed. The software provided can also attempt to document cell regularity etc. Here we compare the outcome of manual vs automatic measurements based on the software linked to the imaging platform.
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Why is it important?
Flow cytometry is a critical method for assessment and analysis of cell types in biological samples, however, imaging of the cells help to further increase the reliability of results, since debris and other particles may be counted automatically in the absence of evaluation of the particle size, shape and intracellular location of the immunofluorescence staining.
Perspectives
This is an interesting way to evaluate suspended cells in a high throughput way. This area may lead to other development using deep learning and AI, etc.
Prof Louis Tong
National University of Singapore
Read the Original
This page is a summary of: Quantitative Image Analysis of Cellular Morphology Using Amnisî ImageStreamX Mark II Imaging Flow Cytometer: A Comparison against Conventional Methods, Single Cell Biology, January 2015, OMICS Publishing Group,
DOI: 10.4172/2168-9431.s1-001.
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