Antimicrobial Sensitivity, Biochemical Characteristics and Biotyping of Staphylococcus saprophyticus: An Impact of Biofield Energy Treatment

What is it about?

Staphylococcus saprophyticus (S. saprophyticus) is a frequent cause of urinary tract infection in the young women. The current study was designed to analyze the effect of biofield energy treatment on S. saprophyticus for evaluation of its antibiogram profile, biochemical reactions pattern and biotyping characteristics. Two sets of ATCC samples were taken in this experiment and denoted as A and B. Sample A was revived and divided into two parts Group (Gr.I) (control) and Gr.II (revived); likewise, sample B was labeled as Gr.III (lyophilized). Gr. II and III were given with Mr. Trivedi’s biofield energy treatment. The control and treated groups of S. saprophyticus cells were tested with respect to antimicrobial susceptibility, biochemical reactions pattern and biotype number using MicroScan Walk-Away® system. The 50% out of twenty-eight tested antimicrobials showed significant alteration in susceptibility and 36.67% out of thirty antimicrobials showed an alteration in minimum inhibitory concentration (MIC) value of S. saprophyticus in revived treated cells (Gr. II, day 10), while no alteration was found in lyophilized treated cells (Gr. III, day 10) as compared to the control. It was also observed that overall 14.81%, out of twenty-seven biochemical reactions were altered in the revived treated group with respect to the control. Moreover, biotype number was changed in Gr. II, on day 5 (246076) and in Gr. III, on day 10 (242066), while organism along-with biotype number was also changed in Gr. II, on day 10 (342066, Staphylococcus hominis subsp. novobiosepticus) as compared to the control (242076, S. saprophyticus). The result suggested that biofield treatment has the significant impact on S. saprophyticus in revived treated cells with respect to the antimicrobial susceptibility, MIC, biochemical reactions pattern and biotype.

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Why is it important?

Staphylococcus saprophyticus (S. saprophyticus) is a Gram-positive, coagulase-negative facultative bacterium belongs to Micrococcaceae family. It is a unique uropathogen associated with uncomplicated urinary tract infections (UTIs), especially cystitis in young women. Young women are very susceptible to colonize this organism in the urinary tracts and it is spread through sexual intercourse. S. saprophyticus is the second most common pathogen after Escherichia coli causing 10-20% of all UTIs in sexually active young women [1-3]. It contains the urease enzymes that hydrolyze the urea to produce ammonia. The urease activity is the main factor for UTIs infection. Apart from urease activity it has numerous transporter systems to adjust against change in pH, osmolarity, and concentration of urea in human urine [2]. After severe infections, it causes various complications such as native valve endocarditis [4], pyelonephritis, septicemia, [5], and nephrolithiasis [6]. About 150 million people are diagnosed with UTIs each year worldwide [7]. Several virulence factors includes due to the adherence to urothelial cells by release of lipoteichoic acid is a surface-associated adhesion amphiphile [8], a hemagglutinin that binds to fibronectin and hemagglutinates sheep erythrocytes [9], a hemolysin; and production of extracellular slime are responsible for resistance properties of S. saprophyticus [1]. Based on literature, S. saprophyticus strains are susceptible to vancomycin, rifampin, gentamicin and amoxicillin-clavulanic, while resistance to other antimicrobials such as erythromycin, clindamycin, fluoroquinolones, chloramphenicol, trimethoprim/sulfamethoxazole, oxacillin, and penicillin [10]. An alternative i.e., biofield energy based healing therapy is recently reported to alter the antimicrobial sensitivity pattern in different microorganisms [11]. Biofield (putative energy fields) or electromagnetic based energy therapies, used to promote health and healing had been exclusively reported by National Institute of Health/National Center for Complementary and Alternative Medicine (NIH/NCCAM) [12]. The human body naturally emits the waves in the form of bio-photons, which surrounds the body and it is commonly known as biofield. In the recent year, Prakash et al. reported that various scientific instruments such as Kirlian photography, polycontrast interference photography and resonance field imaging can be extensively used to measure the biofield of human body [13]. Although, a human has the capability to harness the energy from the environment or universe and can transmit it into any object(s) around the Globe. The objects always receive the energy and responding in a useful way that is called biofield energy and the process is called as biofield treatment. Mr. Trivedi’s unique biofield energy treatment (The Trivedi Effect®) has been known to alter the characteristics features of pathogenic microbes [14,15], an improved growth and productivity of plants [16,17] and also able to alter the thermophysical properties of metal and ceramic in materials science [18,19]. Due to the clinical importance of S. saprophyticus and literature reports on biofield, this work was undertaken to evaluate the impact of biofield treatment in relation to the antimicrobials susceptibility and biotyping based on various biochemical characteristics.

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