Progress in high-sensitivity hybrid LC–MS/MS methods for the bioanalysis of protein drugs and performance tests for their validation

What is it about?

Over the past decade LC-MS has been used increasingly to quantify proteins in biological fluids. The preferred method for proteins > 10 KDa utilizes proteolytic digestion to release component peptides, then LC-MS/MS to quantify a surrogate peptide. The sensitivity can be amplified by using immunocapture to pre-purify the analyte protein and/or the surrogate peptide after digestion. Special techniques are being developed to validate and trouble shoot immunocapture methods. The benefits of these techniques are briefly described in this editorial.

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