Method to create a preclinical free skin flap model in rats with ischemia-reperfusion injury.

What is it about?

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery.

Featured Image

Why is it important?

The ischemia-reperfusion injury-related damages can be studied in this model, making it a suitable model for evaluating therapeutic agents to address this pathophysiological process. Furthermore, two main monitoring techniques are described in the protocol for the assessment of this animal model: transit-time ultrasound technology and laser speckle contrast analysis.