What is it about?
A new phthalylglycyl chloride was proposed as a derivatization agent for the sensitive determination of biogenic amines including octopamine, adrenaline and dopamine determination in urine. The derivatization procedure facilitated the use of reversed-phase HPLC with positive electrospray ionization–high-resolution mass spectrometry (HRMS). An HPLC-HRMS method was developed that provided quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes. The 95–97% yield of derivates was observed after a 10 min derivatization with phthalylglycyl chloride at pH 6.5 and 30 °C.
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Why is it important?
The determination of catecholamines in biological fluids is an important analysis, so the new sensitive methods is proposed. The application of derivatization agents is normally improves sensitivity and selectivity, simplifies preconcentration and detection of derivatives.
Perspectives
It would be useful to expand the class of biogenic amines, which can be detected with help of this reagent.
Professor Pavel N Nesterenko
M.V. Lomonosov Moscow State University
Read the Original
This page is a summary of: Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine, Molecules, March 2023, MDPI AG,
DOI: 10.3390/molecules28072900.
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