What is it about?
Bacteria can create sticky biofilms that make surfaces around water unpleasantly wet. This paper introduces an easy method to measure these biofilms using photos and a software called ImageJ. We hope this approach will help develop better ways to control and remove biofilms.
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Photo by CDC on Unsplash
Why is it important?
With the expected establishment of an international standard for biofilm quantification by the SIAA in Japan in 2023, the method proposed in this paper is crucial. It offers a practical and accessible option for accurately measuring biofilms, which could play a key role in the development of standardized and effective biofilm control strategies.
Perspectives
Working on this paper has been a rewarding experience, as it allowed me to explore a novel approach to biofilm quantification through the color reflection from materials. This method has the potential to simplify and standardize the evaluation of biofilms, particularly when compared to traditional staining techniques like crystal violet extraction. I believe this approach could be widely adopted and make a significant impact in both research and practical applications. It’s exciting to think that this work might contribute to advancing the field of biofilm research and potentially influence future international standards.
Professor Hideyuki Kanematsu
National Institute of Technology, Suzuka College
Read the Original
This page is a summary of: Quantitative Analyses of Biofilm by Using Crystal Violet Staining and Optical Reflection, Materials, September 2022, MDPI AG,
DOI: 10.3390/ma15196727.
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