What is it about?
Proteins such as bovine serum album and glucose isomerase are commonly used as small angle scattering protein size standards. EAch have specific problems.This paper describes the development of a new protein standard for small angle scattering experiments, specifically monomeric green fluorescent protein. The protein is an adaption of green fluorescent protein, which is essentially monomeric at SAS concentrations. The protein is characterised using small angle neutron and x-ray scattering and analytical ultracentrifugation.
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Why is it important?
The standardization of protein small angle scattering experiments and results is becoming increasing important, as the instrumentation, hardware and software develop.
Perspectives
This works links small angle x-ray and neutron experiments, alongside analytical ultracentrifugation. It highlights the advantages of each technique and characterises monomeric GFP in comparison to standard GFP.
Daniel Myatt
Read the Original
This page is a summary of: Monomeric green fluorescent protein as a protein standard for small angle scattering, Biomedical Spectroscopy and Imaging, December 2017, IOS Press,
DOI: 10.3233/bsi-170167.
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