DNA Sequencing of ESBL encoding BlaVEB Gene

What is it about?

Among ESBLs, the most widespread and clinically relevant classes are the TEM, SHV, and CTX-M types. The TEM and SHV types are derived from the TEM-1, TEM-2, and SHV-1 penicillinases. Other plasmid-mediated ESBLs, such as PER, VEB, GES, and IBC β-lactamases, have been described but are uncommon and have been found mainly in Pseudomonas aeruginosa and at a limited number of geographic sites. The blaVEB group is one of the smaller subsets of the class A β-lactamases. The blaVEB enzymes appear to be frequently observed in non-fermenting species such as P. aeruginosa, Acinetobacter baumannii, and other Enterobacteriaceae spp., and their rate of proliferation is increasing.

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Photo by National Cancer Institute on Unsplash

Photo by National Cancer Institute on Unsplash

Why is it important?

This study aims to investigate the molecular basis of resistance to modern β-lactams by ESBLs encoded by the blaVEB gene and their sequencing. PCR was performed on extracted blaVEB genes and sequencing of the target PCR products was performed. All blaVEB sequences were compared with the available sequence data in ESBL producing 135 bacterial isolates. The blaVEB gene plays an essential role in the resistance of ESBL-producing isolates to new β-lactams. Further, the sequencing and phylogenetic analyses of the genes from the P. aeruginosa, K. pneumonia, and E. coli isolates revealed 99% similarity with the GenBank global standard genes.

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