What is it about?
MspA from Mycobacterium smegmatis has become a viable tool for the design of nanobiosensors and bioelectronic devices. However, the classic production method of MspA is prohibitively expensive. Here, we present a cost-effective method of growing MspA in M. smegmatis, followed by extraction and purification.
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Why is it important?
MspA is a homo-octameric protein that is funnel-shaped and ultrastable. This enables numerous applications in nanobiotechnology. The only setback - to date - is that MspA is prohibitively expensive. We were able to grow M. smegmatis in a much more inexpensive cell culture medium than previously used.
Perspectives
I was privileged to work together with Dr. Niederweis (UAB) on the structure and function of MspA since 1999. This protein has become a really important tool for advanced DNA sequencing and numerous other applications.
Stefan Bossmann
Kansas State University
Read the Original
This page is a summary of: Adaptation of Mycobacterium smegmatis to an Industrial Scale Medium and Isolation of the Mycobacterial PorinMspA, The Open Microbiology Journal, May 2013, Bentham Science Publishers,
DOI: 10.2174/1874285801307010092.
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