What is it about?
summery abstract: The interleukin-1 family has multifaceted roles in men٫s reproductive system. Out of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased. The current study aims to verify a possible linkage of Variable Number Tandem Repeat (VNTR) polymorphism of the IL-1RN gene with human men infertility. The study groups enrolled included 100 infertile men and 100 fertile and healthy men. Their seminal fluids were subjected to analysis. Also peripheral blood samples were collected for the assessment or detection of polymorphic Variable Number Tandem Repeats (VNTR) polymorphism of interleukin-1 receptor antagonist gene (IL-1RN). Two alleles, namely IL-1RN1 allele corresponding to 410bp fragment and IL-11RN2 that corresponding to 240bp fragments, are a marker for human men infertility, detected by PCR technique. The results delineated a high frequency of IL-1RN2 allelic gene variants (26%), and two VNTR allelic gene variants carriers IL-1RN1 and IL-1RN2 (16%) among infertile men with significant impacts on sperm motility and morphology (P< 0.000-0.002) respectively. This prospective study in Kurdistan region (Erbil – Iraq) defined a significant impact of VNTR polymorphism of IL-1RN gene in the etiology of men infertility especially on sperm motility and morphology; particularly carriers of IL-1RN2 allelic variants.
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Why is it important?
In summary, we concluded that the VNTR polymorphism of IL-1RN gene had an important role in the etiology of men infertility particularly on sperm motility and morphology
Perspectives
Yasin K. Amin1* Ashti M. A. Said1 Ahmed A. Al-Naqshbandi2 . Saeed Ghulam3 Detection of IL-1RN VNTR Gene Polymorphism Whole blood was taken from the study groups and collected in EDTA tube (2.5 ml) then kept at - 20cº. Detection of VNTR of the IL-1RN gene polymorphism from the collected blood samples was assessed by using the polymerase chain reaction technique carried out at the Medical Research Center/Hawler Medical University in Erbil City/ IRAQ. Briefly, DNA was isolated from the blood samples (100 prep Fermentas DNA extraction kit), and the polymorphic region was amplified by PCR (Bioron / Genekam Biotechnology ready to use PCR master mix). The extracted DNAs were subjected to thirty cycles, (the protocol followed for PCR was initiation denaturation for five minutes at 94˚C followed by 30 cycles of denaturation for 40 second at 94˚C, then annealing for 40 second at at 56˚ C, followed by an extention for 40 second at 72˚C and a final extention at 72˚Cfor five minutes after 30 cycles). The amplification of IL-1RN VNTR Gene Polymorphism was done with forward primes 5-CTCAGCAACACTCCTAT-3 and reverse primer 5-TCCTGGTCTGCAGGTAA-3. The PCR products were analyzed by 2 % agarose gel electrophoresis for 55 minutes at 97 V. (Genekam Biotechnology AG, Germany). The samples were scored as positive for IL-1RN1 VNTR allele corresponding to a 410-bp fragment and 240 bp fragment corresponding to IL-1RN2 VNTR allele.
Prod.Dr.Yasin Kareem Amin yasin amin
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This page is a summary of: Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq, Baghdad Science Journal, September 2021, College of Science for Women,
DOI: 10.21123/bsj.2021.18.3.0438.
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