What is it about?
Morpho–Metrical and Stereological Analysis of Human Fetal Kidney During Mid-Gestation Period Ranging
Featured Image
Photo by National Cancer Institute on Unsplash
Why is it important?
Morpho–metrical and stereological analysis of human fetal kidney during mid-gestation period ranging from 12th to 35th weeks of gestation to study growth pattern of kidney in qualitative and quantitative aspects. Aims and objective: Carry out Morpho-metrical Analysis of human fetal kidney during midgestation period ranging from 12th to 35th week and evaluating following parameters: a. Physical dimensions and weight of each kidney. b. Glomerular area for each kidney at different gestational ages. c. The numerical density of glomeruli in the cortex of each kidney at different gestational ages. d. Volume of glomeruli in the 3 different zones of each kidney at different gestational ages. Methods: For study purpose we divided 31 aborted fetuses into six groups according to gestational age. After measuring external parameters such as CRL of fetuses; they were injected with 10% formalin and fixed in 10% formalin for 3 days. Kidneys were procured; physical dimensions, volume and weight of kidney were measured. After tissue processing, blocks were cut; sections were mounted and H&E stained. Using software analyzer density, area, volume of glomeruli at three different zones i.e. cortex, juxtramedullary and medullary regions were calculated. Glomerular area and volume of cortical, juxtamedullary and medullary region for each kidney at different gestational ages was estimated by measuring the area and volume of 100 glomeruli per fetal kidney under 20X objective lens of image analyzer. Comparison was done for three different zones.
Perspectives
Morpho–metrical and stereological analysis of human fetal kidney during mid-gestation period ranging from 12th to 35th weeks of gestation to study growth pattern of kidney in qualitative and quantitative aspects. Aims and objective: Carry out Morpho-metrical Analysis of human fetal kidney during midgestation period ranging from 12th to 35th week and evaluating following parameters: a. Physical dimensions and weight of each kidney. b. Glomerular area for each kidney at different gestational ages. c. The numerical density of glomeruli in the cortex of each kidney at different gestational ages. d. Volume of glomeruli in the 3 different zones of each kidney at different gestational ages. Methods: For study purpose we divided 31 aborted fetuses into six groups according to gestational age. After measuring external parameters such as CRL of fetuses; they were injected with 10% formalin and fixed in 10% formalin for 3 days. Kidneys were procured; physical dimensions, volume and weight of kidney were measured. After tissue processing, blocks were cut; sections were mounted and H&E stained. Using software analyzer density, area, volume of glomeruli at three different zones i.e. cortex, juxtramedullary and medullary regions were calculated. Glomerular area and volume of cortical, juxtamedullary and medullary region for each kidney at different gestational ages was estimated by measuring the area and volume of 100 glomeruli per fetal kidney under 20X objective lens of image analyzer. Comparison was done for three different zones.
Red Flower Publication Publications
Red Flower Publication Pvt Ltd
Read the Original
This page is a summary of: Morpho–Metrical and Stereological Analysis of Human Fetal Kidney During Mid-Gestation Period Ranging from 12th to 35th Weeks to Study Growth Pattern of Kidney in Qualitative and Quantitative Aspects, Indian Journal of Anatomy, October 2020, Red Flower Publication Private, Ltd.,
DOI: 10.21088/ija.2320.0022.9420.2.
You can read the full text:
Contributors
The following have contributed to this page
