Biofield Energy Treated Vitamin D3 on the Structural Crosslinks, Maturation, and Bone Mineralization

What is it about?

The objective was to investigate the potential of Biofield Treated vitamin D3 and DMEM on bone markers. The test items (TIs) were separated into two parts. One part of each sample was received Consciousness Energy Treatment by Alice Branton and termed as Biofield Treated (BT) samples, while other parts of each sample denoted as untreated TI (UT). MTT data found TIs were safe and nontoxic at tested concentrations. ALP was significantly elevated by 390% and 200% in UT-DMEM + BT-TI at 0.1 and 1µg/mL, respectively; while, increased by 135.29% in BT-DMEM + BT-TI at 0.1µg/mL than UT-DMEM + UT-TI. ALP was significantly increased by 106.34%, 138.47%, and 212.68% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 10µg/mL than untreated. Collagen was significantly increased by 288.68% and 106.96% in UT-DMEM + BT-TI and BT-DMEM + BT-TI, respectively at 10µg/mL than untreated. Further, collagen was significantly increased by 435.78% and 139.72% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 50µg/mL than untreated. Percent of bone mineralization was significantly increased by 299.13%, 239.91%, and 218.77% in BT-DMEM + BT-TI at 0.1, 1, and 10µg/mL, respectively than untreated. Besides, percent of bone mineralization was distinctly increased by 192.51% in BT-DMEM + UT-TI at 10µg/mL than untreated. Overall, Biofield Treated vitamin D3 remarkably improved bone parameters in MG-63 cells and could be an alternative to vitamin D3 deficiency and used various bone disorders viz. low bone density, osteoporosis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

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Why is it important?

Vitamin D has multiple effects in different organs viz. liver, heart, brain, kidneys, lungs, etc. It also possess a significant anti-aging, anti-inflammatory, anti-osteoporosis, anti-stress, anti-arthritic, anti-apoptotic, anti-cancer, wound healing, anti-fibrotic actions and anti-psychotic [1]. Vitamin D receptors (VDRs) are present in different organs viz. liver, brain, kidneys, heart, pancreas, lungs, muscles, large and small intestines, nervous system, reproductive, etc. VDRs can influence communication from one cell to another cell, neurotransmission, cell growth and differentiation, cell cycling and proliferation, immune and cardiovascular functions, hormonal balance, skin health, etc. Most of the living vertebrates, vitamin D is essential for maintaining a good skeletal structure and bone. Naturally, it is synthesized in presence of sunlight in the skin [2]. Most of foods vitamin D is almost absent. However, in current scenario due to several factors like aging, over use of sun protectives, and also due to alteration of zenith angle of sun, etc. the production of vitamin D3 has drastically reduced [3]. It was well established that aging causes bone marrow depression and also reduced muscle strength, that ultimately great impact on the immune and inflammatory responses [4]. Deficiency of vitamin D3 causes metabolic bone diseases like osteomalacia and exacerbate osteoporosis, etc. [5]. The quality of life for menopause women is one of the most critical health problem in the today world. The metabolic bone disorder like osteoporosis is mainly prevalent in post-menopausal women. Due to hormonal factors and a rapid bone loss in post-menopausal women that lead to an increased risk of fracture of bones [6]. Therefore, the serum calcium and alkaline phosphatase (ALP) levels are the two most suitable markers in post-menopausal women for the assessment of bone metabolism. Besides, bone-specific ALP is also an important marker for osteoblast differentiation [7]. Further, increased calcium intake along with adequate supply of vitamin D is important for maintaining a good bone structure. Vitamin D also further responsible for maintaining an adequate level of serum calcium and phosphorus [8-9]. The strength of bone depends on the microarchitecture, quality, mineral content, and collagen content. Collagen is the primary structural protein mainly responsible for calcification of bone. In aged peoples, the mechanical properties of bone became reduced and the bone get became fragile, that causes various bone-related disorders associated with bone-collagen-abnormalities and bone fragility, such as osteogenesis imperfecta and osteoporosis [10-11]. In recent years, several scientific reports and clinical trials have revealed that the useful effects of the Biofield Energy Treatment, which has shown enhanced immune function in cases of cervical cancer patients with therapeutic touch [12], massage therapy [13], etc. Complementary and Alternative Medicine (CAM) therapies are now rising as preferred models of treatment, among which Biofield Therapy (or Healing Modalities) is one approach that has been reported to have several benefits to enhance physical, mental and emotional human wellness. However, the National Health Interview Survey (NHIS), 2012, reported approximately 20% Americans used dietary supplement as complementary health approached compared with conventional therapeutics. The National Center of Complementary and Integrative Health (NCCIH) has supported Biofield Energy Healing is a types of CAM health care approach in addition to other therapies, medicines and practices such as natural products, Qi Gong, Reiki, deep breathing, special diets, yoga, Tai Chi, homeopathy, chiropractic/osteopathic manipulation, acupuncture, meditation, massage, guided imagery, progressive relaxation, relaxation techniques, acupressure, healing touch, hypnotherapy, movement therapy, rolfing structural integration, pilates, Ayurvedic medicine, naturopathy, mindfulness, traditional Chinese herbs and medicines, aromatherapy, essential oils, and cranial sacral therapy. Human Biofield Energy has subtle form of energy that has the ability to work in an effective manner [14]. This energy can be harnessed from the environment and transmitted by the experts into both living and non-living things via the process of Biofield Energy Healing Treatment. CAM therapies have been practiced worldwide with reported many clinical benefits in different disease conditions [15]. Biofield Energy Treatment (The Trivedi Effect®) has been published in numerous peer-reviewed science journals and contributes the significant outcomes in many scientific fields such as cancer research [16-17], microbiology [18-19], biotechnology [20-21], pharmaceutical science [22-25], agricultural science [26-27], materials science [28-31], nutraceuticals [32,33], skin health [34,35], bone health [36-37], human health and wellness. Based on the literature information and importance of vitamin D3 on bone health, authors designed a study to evaluate the impact of Biofield Energy Treatment (The Trivedi Effect®) on the test samples (vitamin D3 and DMEM) for bone health activity with respect to ALP, collagen content, and bone mineralization using standard assay in MG-63 cells.

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