What is it about?
An experiment was carried out by combining the enzymatic assay using fl uorogenic substrates with microscopical counts obtained using the viability marker 5-cyano-2,3 ditolyl-tetrazolium chloride (CTC). The main objective was to verify whether the enzyme activity played by microbial cells depends of the cell viability or rather whether the hydrolytic activity is directly stimulated by the availability of organic substrates for microbial metabolism.
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Why is it important?
This is the first contribution to link the enzyme activity rates with the viability properties (in terms of actively metabolising components) of bacterioplankton inhabiting pelagic Mediterranean waters.
Perspectives
In the examined oligotrophic waters the enzymatic activities seem to be stimulated mostly by the availability of metabolisable organic substrates
Dr Gabriella Caruso
Consiglio Nazionale delle Ricerche, Istituto di Scienze Polari (CNR-ISP) Messina, Italy
Read the Original
This page is a summary of: Microbial enzymatic activity measurements by fl uorogenic substrates: Evidence of inducible enzymes in oligotrophic Mediterranean areas, Journal of Clinical Microbiology and Biochemical Technology, November 2019, Peertechz.com,
DOI: 10.17352/jcmbt.000034.
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