Indole compounds in mycelial cultures of edible mushrooms

What is it about?

Agaricus bisporus and Imleria in vitro cultures were cultivated on modified Oddoux medium, and Oddoux medium enriched with serine or anthranilic acid. Determination of indole compounds in the obtained biomass was carried out using TLC with densitometric detection. Agaricus bisporus with addition of anthranilic acid or serine to the medium at the concentration of 0.5 g/L was the most beneficial. In case of Imleria badia, anthranilic acid at the concentration of 0.5 g/L was the most optimal. The study indicates that modification of the medium can provide satisfactory results and it is worth to search for its new, improved compositions.

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Why is it important?

This is a first report that shows that medium modification through serine or anthranilic acid supplementation affects the content of indole compounds in the resulting biomass. The concentration of the additive added to the medium is also important in the context of quantitative and qualitative content of these compounds in the resulting biomass. It indicates that modification of medium for mycelial cultures may be crucial for the potential production of lyophilized biomass containing specific indole compounds at suitable amounts that can be used in the supplementation of indole derivatives.

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