Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels

What is it about?

The increasing interest and clinical relevance of vitamin D demands analytically reliable and cost-effective testing methods. 25-hydroxyvitamin D (25(OH)D), is widely accepted as the best indicator of the individual vitamin D status. In routine clinical laboratory testing, 25(OH)D immunoassays still play an important role and many new automated immunoassays have been introduced. Several studies, however, have demonstrated that 25(OH)D measurements performed with many immunoassays still suffer from 25(OH)D underestimation that are vitamin D Binding Protein concentration dependent, when compared to liquid chromatography/tandem mass spectrometry. In this manuscript we demonstrate that a relatively simple pepsin pretreatment prior to 25(OH)D analysis on the Lumipulse immunoanalyser almost completely corrects this underestimation of 25(OH)D measurement in subjects with high vitamin D binding protein levels, i.e. pregnant women.

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