Strong impact on plasma protein profiles by precentrifugation delay but not by repeated freeze-thaw cycles, as analyzed using multiplex proximity extension assays

What is it about?

We applied multiplex proximity extension assays to measure the effects on plasma proteins by pre-centrifugation delay of blood, and by repeated freeze-thaw cycles of the plasma samples. We show that plasma should be isolated within three hours at a refrigerated temperature after blood is drawn to avoid artificial deviations of protein levels. By contrast, the impact of plasma proteins by several freeze-thaw cycles was negligible.

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