Assessment of Energy of Consciousness Healing on Cell Growth of Plant Stem Cells Callus Culture

What is it about?

The present study was aimed to evaluate the effect of Consciousness Energy Healing Treatment on plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for its growth and yield on day 7. The plant callus of all the three plants were divided into two parts, one part received Biofield Energy Treatment remotely (known as The Trivedi Effect®-Energy of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Gopal Nayak and denoted as Biofield Energy Treated group, while another part did not receive any treatment and defined as control. Plants callus were observed on day 7 after treatment and visualized under phase contrast microscope for cell growth, captured photomicrographs along with wet weight compared with day 0. Results suggested that three plants viz. Mandukparni, Katsarika, and Amla callus after 7 days showed a significant improved growth rate as compared with the respective untreated groups. The weight of the callus growth (in mg) after Biofield Energy Treatment among Mandukparni, Katsarika, and Amla callus on day 7 was 630, 782, and 920 mg, respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika and Amla was 11.1%, 52.4%, and 68.5%, respectively than untreated. Hence, results concluded a significant growth in callus weight after Biofield Energy Treatment, which suggested that it could be used as complementary and alternate approach in order to produce most high-value phytoconstituents from plant callus that can be used for making various drugs, food flavoring and colouring agents.

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Why is it important?

Plant tissue culture also defined as cell culture, sterile culture, in vitro, and axenic is one of the vital tool now a days in basic and applied science research studies, which have been implicated in various commercial application [1]. Under defined in vitro physical and chemical conditions, plant cell tissue culture is maintained in an aseptic conditions from cells, tissue, organ, and their associated components. These cultures are very useful for commercial applications for its secondary metabolites, which can be directly used as drug or as one of the major constituents of medicinal agent. The plant callus cultures are one of the best methods for the conservation of medicinal plants, which provide high-value natural products (NPs) such as codeine, nicotine, strychnine, azadirachtin, morphine, and paclitaxel [2]. Besides, they are also used as flavoring and coloring agents using various approved commercial methods. Chemical synthesis of metabolites is one of the alternative and costly approaches, which is also associated with the toxic by-products [3]. Thus, plant tissue culture can be considered as the best approach and well-established platform for NPs synthesis with various advantage and wide therapeutic application [4, 5]. The present experimental study assessed the growth and yield of plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata) and Amla (Phyllanthus emblica) after treatment with The Trivedi Effect®-Energy of Consciousness Healing Treatment by a renowned Biofield Energy Healer, Gopal Nayak. Centella asiatica L. also known as Indian pennywort, it has been reported for multiplication of its callus through shoot tip culture [6] and meristem tip culture [7]. Besides, this plant has significant benefits used for asthma treatment, skin diseases, nervine tonic, hypertension, dropsy, bronchitis, and urethritis (inflammation of the urethra) [8]. Phyllanthus emblica Linn. (Indian gooseberry or amla) significantly used as alternative medicine in order to boost the vitality and vigor, along with its significant action as anti-inflammatory, wound healing, antianemia, cardioprotective, antipyretic, anti-diarrheal, analgesic, and many more [9]. Similarly, Barleria cristata, a perennial shrub has been reported to have many biological active compounds viz. iridoids, flavonoids, and phenylethanoid derivatives against many biological activities [10]. The potential mechanisms of certain Energy-Healing practices have been increased from many decades in different biological fields. As a result, healing practitioners (The Trivedi Effect®) have been involved in a wide number of preclinical, in vitro cell lines, analytical, pharmaceutical, agricultural studies. Complementary and Alternative Medicine (CAM) has also defined and recognized Biofield Energy Healing with significant results in biological studies [11]. National Center for Complementary and Alternative Medicine (NCCAM) also mentioned about the importance of Biofield therapies in the subcategory of Energy Therapies. Hence, Biofield Energy Healing is increasingly accepted to promote human wellness as an alternate approach [12, 13]. The Trivedi Effect®-Consciousness Energy Healing Treatment has been reported with significant revolution in the physicochemical properties of metals [14-17], improved agricultural crop yield, productivity, and quality [18-20], transformed antimicrobial characteristics of pathogenic microbes at genetic level [21-23], improved biological activity of nutraceutical compounds for skin health [24, 25], livestock [26], biotechnology [27, 28], improved bioavailability [29-31], nutraceuticals [32, 33], cancer research [34, 35], bone health [36-38], human health and wellness.

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