Assessment of Biofield Energy Healing Based Vitamin D3 Effects on Bone Health Parameters

What is it about?

Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.

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Why is it important?

Bones play an important role for skeletal structure, organ protection, muscles anchor, and storing excess calcium for future utilization. Bones are always changing i.e., new bones are forming and old bones are decaying. In certain condition, when bone decaying is higher than bone formation leads to various bone-related disorders like osteoporosis, osteomalacia, etc. [1]. Osteoporosis is a progressive metabolic bone disorder characterized by reduced bone mass and increased chances of bone fractures [2]. Postmenopausal osteoporosis is a major health issue for women. A numbers of factors are responsible for the increased incidence of osteoporosis such as lifestyle, diets, ethnicity, smoking, alcoholism, and diet have been linked with an increased incidence of the osteoporosis [3]. For prevention of bone-related disorders and to maintain a good bone health usually required optimum ingestion of calcium (Ca). Although, increased level of bone density after adequate supplementation of Ca is not always impact on bone strength and low risks from fracture [4-6]. Vitamin D is a hormone acts on vitamin D receptors (VDRs), present in various vital organs like heart, brain, lungs, kidney, liver, etc. and essential for absorption of calcium and bone mineralization which is directly linked with the bone mineral density (BMD) [7]. Deficiency of vitamin D3 causes rickets in children and osteomalacia in adult’s primarily. Literature reported that vitamin D prevent fractures through muscle function and inflammation [8]. A number of experiment reported the role of bone health using cell lines and analyzing the bone -related parameters like alkaline phosphatase (ALP), collagen and bone mineralization in terms of calcium deposition [9]. MG-63 cell line (an immature osteoblast phenotype) is derived from juxtacortical osteosarcoma. The response of MG-63 cells to the active metabolites of vitamin D3 i.e., 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) administration has been studied to be similar to normal human osteoblast cells [10]. Hence, MG-63 cell line is used extensively for the evaluation of bone health parameters [11]. For early differentiation and maturation of osteoblasts cells ALP acts as a phenotypic marker. It increases the local concentration of inorganic phosphate (pi) for bone mineralization and hence, it is used an important biomarker for osteogenic activity [12]. Similarly, collagen plays an important role in the formation of bone extracellular matrix (ECM) by providing strength and flexibility [13]. Similarly, calcium phosphate is deposited and gets mineralized (combination of calcium phosphate and hydroxyapatite) and gives rigidity to the bone [14]. Thus, these parameters are very essential to study the bone health in cell lines. Hence, authors evaluated the in vitro effect of the Biofield Energy Treated vitamin D3 on bone health using MG-63 cell line for major biomarkers. Energy medicine is emerging with significant benefits in various scientific fields. The effects of the complementary and alternative medicine (CAM) therapies have great importance, which include Qi Gong, Reiki, Tai Chi, meditation, acupressure, acupuncture, Johrei, yoga, polarity therapy, pranic healing, therapeutic touch, chiropractic/osteopathic manipulation, deep breathing, massage, homeopathy, progressive relaxation, special diets, guided imagery, relaxation techniques, hypnotherapy, pilates, traditional Chinese herbs and medicines, healing touch, Rolfing structural integration, movement therapy, mindfulness, Ayurvedic medicine, etc. [15]. Consciousness Energy Healing Treatment (The Trivedi Effect®) contain a putative bioenergy, which is channeled by a renowned practitioner from a distance to livings and non-livings objects. Biofield Energy Healing as a CAM showed a significant results in biological studies [16]. However, the National Center for Complementary and Alternative Medicine (NCCAM), well-defined Biofield therapies in the subcategory of Energy Therapies [17]. The Trivedi Effect®-Consciousness Energy Healing Treatment has been reported with significant revolution in multiple fields [18-28] and human health and wellness. Based on the outstanding outcomes of Biofield Energy Treatment and vital role of vitamin D3 on bone health, authors performed this experiment to investigate the effects of Biofield Treated vitamin D3 on bone health parameters like ALP, collagen content, and bone mineralization using standard in vitro assays in MG-63 cells.

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