Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules

What is it about?

This is the first publication of work that used purified rRNA to characterise the microbial (bacterial, archaea & eukaryotic cells) simultaneously in a single community. The technique avoids the use of PCR (commonly used to create DNA for sequencing), which can only identify sequences similar to the primers and can introduce alterations in the structure of the population due to PCR efficiencies. Tis paper describes a population in which 40% of the structure would not have been seen using PCR.

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