What is it about?
This paper compares three human kinases' expression and activity levels when expressed in five different bacterial strains. The expression levels and yields varied considerably as the growth conditions changed, and there was a significant difference in the kinase enzyme activity.
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Why is it important?
Most researchers use one or two bacterial strains when expressing a human kinase, mostly the strains they have growing in their labs. Few papers have discussed how shifting from a basic strain to a specialized one helped the expression. This paper systematically analyzed how three different kinases can exhibit different expression, yield, and activity when the strain of bacteria is changed.
Perspectives
Mentoring undergraduate students is as rewarding as it is challenging; seeing their work come together in this paper like a mosaic has been inspiring.
May Abdelaziz
The University of Texas at Tyler
Read the Original
This page is a summary of: Comparative expression of soluble, active human kinases in specialized bacterial strains, PLoS ONE, April 2022, PLOS,
DOI: 10.1371/journal.pone.0267226.
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