OXIDATIVE STRESS AS A MECHANISM OF COMBINED OTA AND CTN TOXICITY IN RAT

What is it about?

OTA and CTN commonly coexist in grains. Aiming to evaluate oxidative stress in OTA+CTN toxicity male Wistar rats were orally treated with two doses of OTA (0.125 and 0.250 mg kg-1 b.w.), CTN (2 mg kg-1 b.w.), and RSV (20 mg kg-1 b.w.), and combined daily during three weeks. Protein carbonyl concentrations were measured in kidneys and liver, catalytic activity of GPx, SOD and CAT and GSH level in plasma, kidneys and liver while MDA concentration was measured in plasma, kidneys, liver and urine. Mycotoxin treatment significantly increased MDA concentration in plasma and kidney and decreased SOD activity in liver. Rats treated with CTN and OTA125+CTN had lower plasma GPx activity. Concentration of GSH in kidney and protein carbonyls in kidney and liver as well as activities of GPx in kidney and liver, SOD activity in kidney and CAT activity in liver were not affected. Protective effect of RSV was observed on GSH in kidney and plasma and MDA in kidney, plasma and urine. Oxidative stress is involved in OTA+CTN toxicity in vivo because such treatment affects parameters of oxidative stress, particularly in plasma. RSV can reduce but not overcome oxidative stress induced by combined OTA and CTN treatment.

Featured Image

Why is it important?

Oxidative stress plays significant role in single and combined OTA and CTN toxicity mechanism; mycotoxin treatments in male Wistar rats evoked different effects in plasma, kidney and liver which is not surprising because the organization of antioxidative defence is tissue- and cell-specific (15). Effect of mycotoxins on oxidative stress is less pronounced in liver because of its elaborate antioxidant defence system (18).

Perspectives