Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies

What is it about?

The present study was undertaken to evaluate the effect of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against skin health parameters using HFF-1, HaCaT, and B16-F10 cells.

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Why is it important?

The results showed that the cell viability by MTT assay exhibited more than 75% cells were viable in all the tested concentrations, indicating that the test formulation was safe and nontoxic. The percent cell proliferation data using BrdU assay was significantly increased by 434.14%, 244.77%, and 268.53% in the UT-DMEM + BT-Test formulation, BTDMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 μg/mL compared to the UT-DMEM + UT-Test formulation group. Further, it was enhanced by 354.67%, 202.79%, and 107.73% in UTDMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 μg/mL with respect to the UT-DMEM + UT-Test formulation group. At 35 μg/mL the percentage of cell proliferation was elevated by 360.43%, 326.18%, and 327.95% in the UT-DMEM + BT-Test formulation, BTDMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the UTDMEM + UT-Test formulation group. The level of elastin was significantly (p≤0.001) increased by 93.52% and 75.81% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 μg/mL compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was increased by 100.07% (p≤0.05), 41.60%, and 50.10% at 0.625 μg/mL in the BT-DMEM + UT-Test formulation, BT-DMEM + BT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. However, it was also increased significantly by 30.35%, 38.55%, and 54.45% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BTDMEM + BT-Test formulation groups, respectively at 1.25 μg/mL compared to the UT-DMEM + UT-Test formulation group. Melanin level was reduced by 9.16% in the UTDMEM + BT-Test formulation group at 0.063 μg/mL with respect to the UT-DMEM + UT-Test formulation group. Additionally, the melanin content was also decreased by 10.09% and 6.51% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.125 μg/mL compared to the UT-DMEM + UT-Test formulation group. Protection with respect to UV-B, revealed that the level of cell viability was increased by 7.22%, 5.75%, and 8.15% in the BT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 μg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing results displayed a significant effect on wound closure and cell migration in all the tested groups in HFF-1 cells compared to the untreated group. Overall, the Consciousness Energy Healing Treated test formulation (The Trivedi Effect®) and DMEM have shown significant protective effects on various skin health parameters such as wrinkling, aging, skin whitening, and wound healing. Therefore, the Biofield Energy Healing based herbomineral formulation would be suitable for the development of herbal cosmetics, and it would be useful for the management of wounds and various skin related disorders viz. skin abscess, pimples, cellulitis, impetigo, scabies, syringoma, photosensitivity, urticaria, hives, warts, abscess, callus, acne, chickenpox, eczema, rosacea, seborrheic dermatitis, athlete’s foot, psoriasis, erythema, contact dermatitis, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color etc.