Mouse Dendritic and Splenocyte Cells for Modulation of Pro-inflammatory Cytokines

What is it about?

The study aims to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine dendritic (DCs) and splenocyte cells.

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Why is it important?

Based on these results, it was concluded that the Biofield Energy Treated formulation modulates the DCs and splenocyte cells function. Further, the Biofield Treated formulation significantly inhibited the activity of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β in both the tested immune cells as compared to the untreated test formulation. The results of MTT assay in DCs after 24 hours of treatment incubation with different concentrations of the Biofield Treated formulation suggest a safe concentration range of 1.05 to 105.2 µg/mL, which was tested for estimation of TNF-α. Similarly, MTT assay in splenocyte cells after 48 hours of treatment incubation with the different concentrations of the Biofield Treated formulation in the presence of 0.5 μg/mL LPS, suggest that the concentration range of 0.0000105 to 1.05 µg/mL was found safe with more than 80% cell viability, which were selected for MIP-1α and IL-1β estimation. The Biofield Treated formulation showed significant (p≤0.001) suppression of TNF-α level in DCs cells at all the tested concentration ranges (i.e. 1.05 to 1052.5 µg/mL), while maximum suppression was reported at 5.2 µg/mL by 43.64% as compared with the untreated test formulation. However, the levels of MIP-1α and IL-1β at the tested concentration range (0.0000105 to 10.5 µg/mL) in LPS stimulated splenocyte cells were significantly inhibited. Further, data suggests that inhibition of MIP-1α (26.52%) and IL-1β (35.28%) in the Biofield Treated formulation at 0.000105 and 0.0000105 µg/mL, respectively as compared with the untreated test formulation. Overall, the inhibition of pro-inflammatory cytokines might prevent the over activation of the human immune system. Thus, it may strongly counteract the pro-inflammatory responses, which might prevent immune-mediated tissue damage. Through insight into immunomodulatory cytokines, the Biofield Energy Treated test formulation can provide the innovative strategy to affect the immune system, which could help combat various acute and chronic inflamatory diseases as a better complementary and alternative medicine. So, it can be concluded that The Trivedi Effect®-Biofield Energy Healing (TEBEH) has the capacity to modulate the immunoinflammatory activity of herbomineral formulations with a safe therapeutic index and a faster absorption rate that can enhance the human well-being in the biomedical health care system. This can be ulitized against many acute and chronic inflammatory diseases like Asthma, Chronic peptic ulcer, Tuberculosis, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease, Rheumatoid arthritis, Chronic periodontitis, Ulcerative colitis, Crohn’s disease, Chronic sinusitis, Systemic Lupus Erythematosus, Type 1 Diabetes and Chronic active hepatitis. Furthermore, the Biofield Energy Treatment Therapy applied to the herbomineral formulation can also be utilized for various autoimmune disorders such as Graves’ Disease, Lupus, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Hashimoto Thyroiditis, Addison Disease, Multiple Sclerosis (MS), Myasthenia Gravis, Aplastic Anemia, Reactive Arthritis, Sjogren Syndrome, Alopecia Areata, Fibromyalgia, Vitiligo, Pernicious Anemia, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as to prevent the immune-mediated tissue damage in cases of organ transplants (for example kidney transplants, liver transplants and heart transplants) because of its safe therapeutic index and potential to modulate the immune system and improve overall health and quality of life. Overall, the Biofield Energy Healing Treated test formulation can also be used for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.