Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield

What is it about?

The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health.

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Why is it important?

The experimental data exhibited significantly improved cell viability evaluated using MTT assay. The data showed more than 70% cells were viable in all the tested concentrations such as HFF-1 (1.25 to 10 μg/mL), HaCaT (0.625 to 5 μg/mL), and B16-F10 (10 to 40 μg/mL). BrdU assay indicated 43.63% and 10.97% increase in the cellular proliferation in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. The collagen level was increased by 23.56% and 13.36% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation group respectively, at the concentration of 1.25 μg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly increased by 8.34% in the BT-DMEM + BT-Test formulation at 2.5 μg/mL compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was significantly increased by 2.60% in the BT-DMEM + UT-Test formulation group at 0.625 μg/mL compared to the UT-DMEM + UTTest formulation group. Melanin level was reduced by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the UT-DMEM + UT-Test formulation group at 0.125 μg/mL. Anti-wrinkling effects with respect to UV-B showed 4.17% and 5.88% at 2.5 μg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation group respectively, compared to the UTDMEM + UT-Test formulation group. The wound healing scratch assay indicated a significant migration of fibroblast and keratinocytes cells with increased covered area up to 10.50% and 8.30% in HFF-1 and HaCaT cells respectively after treatment with the Biofield Energy based herbomineral test formulation. Altogether, the Biofield Energy Treated Test formulation (The Trivedi Effect®-Consciousness Energy Healing Treatment) and DMEM have shown significant skin protection action, which suggests its use against wrinkling, aging, skin whitening, and wound healing. Therefore, test formulation could be suitable for prevention and improvement of various skin related disorders as a novel herbal cosmetics. Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing based herbomineral test formulation can also be used in the prevention of temporary and permanent skin disorders, antiaging, improved overall health, and quality of life.