Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri

What is it about?

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.

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Why is it important?

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The genus Providencia is facultatively anaerobic, chemoorganotrophic, and urease-producing Gram-negative, rod-shaped bacterium that are responsible for a wide spectrum of human infections [1, 2]. Providencia rettgeri (P. rettgeri) is motile by peritrichous flagella, belonging to the family of Enterobacteriaceae. The most remarkable biochemical features to characterize its biochemical abilities are positive reactions of urea and catalase, negative reactions of oxidase, hydrogen sulfide and β-galactosidase [3]. The high abundance of P. rettgeri is mainly in the urinary tract of the compromised or catheterized patient that causes gastrointestinal tract infections or traveler’s diarrhea [4, 5]. The virulence factors of P. rettgeri are lipopolysaccharide (LPS) and production of sidrophores, β-lactamase, and urease [6]. Ciprofloxacin, levofloxacin, piperacillin-tazobactam, meropenem, and amikacin are the choice of drugs in P. rettgeri infection. Based on literature, it was reported that the β-lactamase producing P. rettgeri had marked resistance to multiple drugs [7]. Therefore, due to the clinical significance of P. rettgeri, an effective antimicrobial therapy is very needful for human health. An alternative i.e. biofield energy based healing therapy is recently reported to alter the antimicrobial sensitivity pattern in a different microorganism. Biofield (putative energy fields) or electromagnetic based energy therapies used to promote health and healing had exclusively reported by National Institute of Health/National Center for Complementary and Alternative Medicine (NIH/NCCAM) [8]. The human body naturally emits the waves in the form of bio-photons, which surrounds the body and it is commonly known as biofield. Therefore, the biofield consists of an electromagnetic field, being generated by moving electrically charged particles such as ions, molecule, etc. inside the human body. In the recent year, 2015 Prakash et al. reported that the various scientific instruments such as Kirlian photography, polycontrast interference photography (PIP) and resonance field imaging (RFI) can be extensively used to measure the biofield of human body [9]. Although, a human has the capability to harness the energy from environment or universe and can transmit it into any object(s) around the Globe. The objects always receive the energy and respond it into a useful way that is called biofield energy and the process is known as biofield treatment. Mr. Trivedi’s unique biofield energy treatment (The Trivedi Effect®) has been known to alter the characteristics features of pathogenic microbes [10, 11], an improved growth and productivity of plants [12, 13] and also able to alter the thermophysical properties of metal and ceramic in materials science [14, 15]. Due to the clinical significance of this organism and literature reports on biofield treatment, the present work was undertaken to evaluate the impact of biofield treatment modality on P. rettgeri in relation to the antimicrobials susceptibility, biochemical reactions, and biotyping.

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