Mouse Splenocytes After Incubation with Biofield Treated Herbomineral Formulation

What is it about?

The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the formulation when applied to splenocyte cells isolated from mice spleen.

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Why is it important?

The in vitro results of the Biofield Energy Treated and untreated test formulations showed significant modulatory effects on splenocyte cells isolated from mice with respect to the suppression of tested cytokines. All the tested cytokines (TNF-α, MIP-1α, and IL-1β) were reported with immunosuppressive activity with respect to the vehicle control group, while the Biofield Energy Treatment further potentiated the immunomodulation as compared with the untreated test formulation. The results of in vitro splenocyte cells’ viability assay suggest that most of the tested concentration of the Biofield Treated and untreated test formulations showed improved cell viability, except at the higher concentration of 10.53 µg/mL. The cell viability after the Biofield Energy Treatment significantly improved with the Biofield Treated test formulation at concentrations 0.0001053, 0.001053, and 1.053 µg/mL with respect to the untreated test formulation. The results of the cytokines estimation revealed that the Biofield Treated group suppressed the TNF-α, MIP-1α and IL-1β. TNF-α levels, which were significantly inhibited at three tested concentrations of 0.01053, 0.1053, and 1.053 µg/mL by 1.77%, 1.93%, and 3.55%, respectively in the Biofield Treated test formulation as compared with the untreated test formulation. In the case of MIP-1α, both the untreated and Biofield Treated test formulation showed significant suppression, while the Biofield Energy Treatment enhanced the immunosuppressive activity at the concentration 0.01053 µg/mL by 18.47% (p≤0.001) as compared to the untreated formulation. The values of MIP-1α reported in the case of the untreated formulation was 628.94 ± 13.0 pg/mL, while it was decreased to 512.74 ± 1.9 pg/mL in the Biofield Energy Treated test formulation. Similarly, significant inhibition (p≤0.001) of IL-1β secretion was reported in the Biofield Treated test formulation at concentration 0.001053, 0.01053, 0.1053, and 1.053 µg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation. On the basis of the above estimation, it can be concluded that after receiving the Biofield Energy Healing Treatment (The Trivedi Effect®), administered remotely by the seven Biofield Energy Healers to the new herbomineral formulation significantly inhibited the activity of pro-inflammatory cytokines that might prevent the over-activation of the immune system. The Biofield Energy Treated test formulation can be a better complementary and alternative medicine to prevent the immune-mediated tissue damage in cases of organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis (MS), Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Sjogren Syndrome, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease and stress etc. with a safe therapeutic index to improve overall health and quality of life. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.