The Study of Biofield Energy Treatment Based Herbomineral Formulation in Skin Health and Function

What is it about?

The aim of the study was to evaluate the effect of the The Trivedi Effect®- Consciousness Energy Healing Treatment based new proprietary herbomineral formulation and cell medium (DMEM) on various skin health parameters using HFF-1, HaCaT, and B16-F10 cell lines.

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Why is it important?

The current study established the potential application of the Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing Treatment) based test formulation and cell medium with respect to the different skin health parameters using human fibroblasts, human keratinocytes, and mouse melanoma cell lines (i.e. HFF-1, HaCaT, and B16-F10). MTT assay results showed that the test formulation was found to be safe up to 80 μg/mL concentration. Fibroblast proliferation assay in HFF-1 cells showed a significant proliferation rate at 2.13, 8.75, and 17.53 μg/mL by 3.90%, 14.68% and 29.54% in the BT-DMEM + BT-Test formulation group compared to the untreated group. Among tested ECM components, 3.84% to 7.42% increases in collagen were reported compared with the untreated test formulation and DMEM. Similarly, the elastin level was increased by 19.8% and 25.8% in UT-DMEM + BT-Test Formulation (5 μg/mL; p≤0.05) and BT-DMEM + BT-Test Formulation (5 μg/mL; p≤0.01) groups, respectively compared with the UT-Test Formulation and UT-DMEM. In addition, the level of hyaluronic acid was increased by 1.7% in UT-DMEM + BT-Test Formulation group compared with untreated test formulation and DMEM. Besides, melanin synthesis inhibition was found to be in range of 5.79% to 15.21% in different groups, while UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation group showed a maximum inhibition of melanin synthesis by 15.21% and 11.43%, respectively at 0.125 μg/mL compared with the untreated Test Formulation and DMEM in B16-F10 melanoma cell line. An anti-wrinkling effects using UV-B induced stress in HFF-1 cells of the Biofield Treated test formulation showed 7.65% improved cell viability in the BTDMEM + BT-Test Formulation experimental group at 1.25 μg/mL compared with the UT-DMEM + UT-Test formulation group. The wound-healing scratch assay showed a significantly increased cellular proliferation and migration in HFF-1 and HaCaT cells in the Biofield Energy Treated Test Formulation compared with the untreated test formulation and DMEM. Overall, the Biofield Energy Healing (The Trivedi Effect®) based test formulation has shown substantial protective effects on skin disorders such as wound healing, wrinkling, ageing and hyperpigmentation. Therefore, the Biofield Energy based novel herbal pharmaceuticals might be a suitable approach for the development for herbal cosmetics and their formulation, which can improve the overall skin health care with respect to anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, wrinkles, diaper rash, seborrheic dermatitis, chickenpox, measles, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, excessive flushing. Further, the Biofield Energy Healing Treatment can also be used in the prevention of temporary and permanent skin disorders, anti-aging, and in the improvement of overall health and quality of life.