Ratio of 13C, 2H, and 18O in Biofield Energy Treated Ortho and Meta Toluic Acid Isomers

What is it about?

O-Toluic acid (OTA) and m-toluic acid (MTA) are two isomers of toluic acid that act as an important organic intermediates, mostly used in medicines and pesticides. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, (PM+1)/PM and 18O/16O, (PM+2)/PM, in toluic acid isomers using gas chromatography-mass spectrometry (GC-MS). The OTA and MTA samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to biofield energy treatment. The GC-MS spectra of both the isomers showed five m/z peaks due to the molecular ion peak and fragmented peaks of toluic acid derivatives. The isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM were calculated for both the isomers and found significant alteration in the treated isomers. The isotopic abundance ratio of (PM+1)/PM in treated samples of OTA was decreased and then slightly increased upto 2.37% in T2, where the (PM+2)/PM in treated OTA, significantly decreased by 55.3% in T3 sample. Similarly, in case of MTA, the isotopic abundance ratio of (PM+1)/PM in the treated sample showed a slight increase the (PM+2)/PM was decreased by 11.95% in T2 as compared to their respective control. GC-MS data suggests that the biofield energy treatment on toluic acid isomers had significantly altered the isotopic abundance of 2H, 13C, and 18O in OTA and MTA as compared to the control.

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Why is it important?

The distribution of contaminant sources of any molecule on a native or global scale can be understood by determining the isotopic abundance ratio, and characterization of elementary reaction mechanisms [1]. The natural abundance or any kinetic process that leads to the local depletion or enhancement of isotopes in organic molecules can be successfully determined using gas chromatography-mass spectrometry (GC-MS) [2]. Moreover, the rate of chemical reaction may vary with the mass of the nucleus with different isotopic substitutions, which slightly affect the partitioning of energy within the molecules. These deviations from perfect chemical equivalence are termed as isotope effects. The isotopic abundance ratio is commonly reported in terms of atom percent and determined by high resolution mass spectrometry (HRMS spectrometry) [3]. Two toluic acid derivatives, o-toluic acid (OTA) and m-toluic acid (MTA) were taken for mass spectroscopy study. Both of the toluic acid derivatives are widely used as a chemical intermediate to synthesis the insect repellant in the chemical industry, as plastic stabilizer in polymer industry and as a starting material in pharmaceutical products [4, 5]. MTA is used specifically as starting material in the production of 3-carboxybenzaldehyde, 3-methylbenzophenone, 3-benzoylphenylacetic acid, N,N-diethyl-3-methylbenzamide, etc. [6]. They are mostly found in crude oil and artificially synthesized from toluidines and oxidation of ortho and meta xylenes [7]. Besides, toluic acid isomers are biodegradable products and its bioaccumulation potential is very low as compared to their homologs. This chemical is stable to hydrolysis in water in abiotic conditions, however, indirect photo-oxidation may have occurred via hydroxy radicals in the atmosphere. If xylene and toluene were taken with contaminated food they are metabolized mainly to methylbenzoic and benzoic acid respectively, which eventually conjugated with glycine to produce methylhippuric and hippuric acid, then excreted in the urine [8]. The photophysical stability as well as chemical reactivity of toluic acid isomers is the primary concern and could be altered by an alternative approach: Mr. Trivedi’s biofield energy treatment. The properties could be enhanced by utilizing The Trivedi Effect®, a unique biofield energy treatment by Mr. Trivedi which is already known to alter the physical and structural properties of various living and non-living substances [9,10]. The National Center for Complementary and Alternative Medicine (NCCAM) has recommended the use of biofield therapy as a part of complementary and alternative medical therapies (CAM) in the healthcare sector [11]. CAM includes numerous energy-healing therapies, in which the biofield therapy is a form of putative energy medicine that is being widely used worldwide to improve the overall health of human beings. Humans have the ability to harness energy from the environment/universe and can be transmitted to any object (living or non-living) around the globe. This process is called biofield energy treatment. The impact of the Trivedi Effect® has been studied in various research fields like microbiology, biotechnology, and agricultural research [9,10,12-14]. Based on the outstanding results achieved by the biofield energy treatments on ceramics, microbiology, and agricultural research, an attempt was made to evaluate the effect of biofield energy treatment on the isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM in OTA and MTA.

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