Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone

What is it about?

Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray diffractograms (XRD) showed the halo patterns of XRD peaks in both the samples. The particle size analysis exhibited about 4.70% and 17.58% increase in the d50 (average particle size) and d99 (particle size below which 99% particles are present), respectively of treated bio peptone as compared to the control. The surface area analysis revealed the 253.95% increase in the specific surface area of treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis showed the 29.59% increase in the melting temperature of treated bio peptone sample as compared to the control. Thermogravimetric analysis (TGA) showed the increase in onset of degradation temperature by 3.31% in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as O-H stretching from 3066 cm-1 to 3060 cm-1; C-H stretching from 2980, 2893, and 2817 cm-1 to 2970, 2881, and 2835 cm-1, respectively; N-H bending from 1589 cm-1 to 1596 cm-1; C=C stretching from 1533 cm-1 to 1525 cm-1; and P=O stretching from 1070 cm-1 to 1078 cm-1 in treated sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) i.e. at 259 nm and 257 nm in both the control and treated samples, respectively. Overall, the analytical results suggested that Mr. Trivedi’s biofield energy treatment has substantial effect on physicochemical and spectral properties of bio peptone. Owing to this, the treated bio peptone might be more effective as culture medium than the corresponding control.

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Why is it important?

A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell [1]. The culture media can be classified in several categories based on their physical form (solid media or liquid media), and chemical composition (chemically defined or synthetic media and complexed media), etc. In addition, some specific or selective media are also there; the special media contains various chemicals designed to distinguish the microbes by the appearances of their colonies [2, 3]. The selective media contains elements that inhibit the growth of some kinds of microbes and at the same time, it promotes the growth of other microbes [4]. Similarly, the bio peptone is a mixture of enzymatic digest of animal tissues and casein. It is processed cautiously to enhance the nutritive values for proper growth requirement of wide variety of microorganisms. It offers a wide spectrum of amino acids and peptides and therefore can be used in numerous culture media preparation [5]. The bio peptone meets the nutritional requirements, which are not provided by meat peptone or casein hydrolysate individually. It can also be used for the culture of fastidious microorganisms and production of enzymes, antibiotics, and other microbiological origin products [6]. Sterilization process plays a significant role on the quality of culture media. The autoclaving is the standard method of culture media sterilization [7]. However, the heat treatment of complex culture media may result in destruction of the nutrients either by direct thermal degradation or by the chemical reactions among the components [8]. Hence, a technique is required, which can enhance the overall stability of the bio peptone [9]. In the recent past years, the energy therapies have been reported for beneficial effects in several field throughout the word [10]. Biofield energy treatment is one of the energy therapies [11]. The energy medicines have been categorized under the Complementary and Alternative Medicine (CAM) therapies by National Institute of Health (NIH)/National Center for Complementary and Alternative Medicine (NCCAM) [12]. Consciousness is one of the possible mechanisms among the several proposed one to support the biofield energy therapies. It includes healer’s intent to heal, and may interact with the physical realm [13]. Similarly, physical resonance is another theory, which comprises subtle energies. As per the physical resonance theory, the energy can be exchanged between the energy fields of healer and patient [14]. Mr. Mahendra Kumar Trivedi is a renowned practitioner of energy medicine. He can harnesses the energy from universe and transfers it to the object (living or non-living); this procedure is termed as biofield energy treatment (The Trivedi Effect®). The Trivedi Effect® has been studied in the several fields such as agricultural science research [15], microbiology research [16], and biotechnology research [17], etc. In addition, Mr. Trivedi’s biofield energy treatment has been also reported to alter the various physicochemical properties of organic products [18] and organic compounds [19]. Based on the above mentioned literature reports on biofield energy treatment and the uses of bio peptone in the culture media, the present study was designed to investigate the effect of biofield energy treatment on bio peptone. The treated bio peptone was analyzed using several analytical techniques to assess any modification in its physicochemical and spectroscopic properties. The data of treated sample were compared with that of control sample.

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