Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium

What is it about?

SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently, the control and treated samples were analyzed using various analytical techniques. The CHNO analysis showed about 2.16, 4.87, and 5.89% decrease in percent contents of carbon, hydrogen, and oxygen, respectively; while 9.49% increase in nitrogen contents of treated sample as compared to the control. X-ray diffraction (XRD) analysis showed 7.23% decrease in crystallite size of treated sample as compared to the control. The thermogravimetric analysis (TGA) analysis showed the increase in onset temperature of thermal degradation by 19.61% in treated sample with respect to the control. The control sample showed the 48.63% weight loss during the thermal degradation temperature (Tmax) while the treated sample showed only 13.62% weight loss during the Tmax. The differential scanning calorimetry (DSC) analysis showed the 62.58% increase in the latent heat of fusion of treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated SFRE-M showed the alteration in the wavenumber of C-O, C-N and C-H vibrations in the treated sample as compared to the control. Altogether, the XRD, TGA-DTG, DSC, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal and spectral properties of SFRE-M. The treated SFRE-M was more thermal stable than the control SFRE-M and can be used as the better culture media for mammalian cell culture.

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Why is it important?

Medium M-199 is a well-defined nutritional source for cell culture media, developed in 1950 by Morgan et al. [1, 2]. It is the combinations of amino acids, vitamins and other factors, which exhibited the best growth of explanted tissue in the in vitro studies [3]. It was initially designed for nutritional studies to promote the growth of primary chick embryo heart and fibroblast cells in the absence of serum supplement [3, 4]. Although, the M-199 medium is also useful with serum for growth of a wide range of cell types such as non-transformed chicken, monkey, and human cells. The SFRE 199-1 medium (SFRE-M) is the modified form of medium 199, and developed for the growth and maintenance of mammalian cell culture (such as primary baboon kidney cells) [5]. It is obtained by supplementing the medium M-199 with sodium pyruvate, zinc sulfate, and increasing arginine-HCl, cystine, cysteine, L-glutamic acid, L-glutamine, glycine, tyrosine, histidine, and glucose to maximally active nontoxic concentrations [6]. Sterilization process plays a significant role on the quality of culture media. The autoclaving (heat treatment) is the principle method of culture media sterilization [7]. This heat treatment of complex culture media may result to nutrient destruction via the direct thermal degradation or by the chemical reactions between the components [8]. Therefore, an alternate method is required, which can enhance the overall stability of the culture media such as SFRE-M [9]. Recently, the energy healing therapies have been reported for several beneficial effects throughout the word. Biofield energy treatment is one of the energy therapies that has been reported to alter the various physicochemical properties of organic compounds [10] and organic products [11]. The energy medicines have been categorized by National Center for Complementary and Alternative Medicine (NCCAM) under the CAM therapies [12]. There are several proposed mechanism and explanations are offered to support the biofield energy therapies. Consciousness is one of the possible mechanisms, which includes healer’s intent to heal, may interact with the physical realm [13]. Likewise, physical resonance is another theory that includes subtle energies. According to this theory, the energy might be exchange between the energy fields of healer and patient [14]. The healer or practitioner of energy medicine harness the energy from universe and transmit it to the object (living or non-living), which is called biofield energy treatment. Mr. Trivedi is a renowned practitioner of energy medicine and his unique biofield energy treatment is known as The Trivedi Effect® that has been studied in the field of agricultural science research [15], biotechnology research [16], and microbiology research [17], etc. After conceiving the significant impact of biofield energy treatment in different field of science, the present study was attempted to evaluate its impact on the culture media such as SFRE-M culture media. The biofield energy treated SFRE-M was analyzed along with the control sample using several analytical techniques like elemental (CHNO) analysis, X-ray diffractometry (XRD), thermogravimetric analysis-derivative thermogravimetry (TGA-DTG), DSC, and Fourier transform infrared (FT-IR) spectroscopy. The data of treated sample was compared with that of control as well as reported literature data.

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