What is it about?
Within a single infection assay, we have set a microscopical technique to measure both Listeria entry and vacuolar escape
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Why is it important?
This is robust assay that allows now to make perturbations in host cells and determine whether these perturbations affect entry and/or vacuolar escape of Listeria
Perspectives
This technique may be used now to identify host factors that modulate the vacuolar stage of Listeria, either promoting bacterial translocation to the cytoplasm, or inhibiting translocation and favoring bacterial destruction within lysosome-related compartments
Dr Javier Pizarro-Cerda
Institut Pasteur
Read the Original
This page is a summary of: A Dual Microscopy-Based Assay To Assess Listeria monocytogenes Cellular Entry and Vacuolar Escape, Applied and Environmental Microbiology, October 2015, ASM Journals,
DOI: 10.1128/aem.02302-15.
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