What is it about?
Describes the cell based assay of 2 replicon cell lines: Hepatitis C virus and BVDV using read-outs consisting of a FRET protease assay (HCV) and a luciferase reporter (BVDV) assay. A 3rd assay employing Alamar Blue (fluorescent blue indicator dye called resazurin) is used prior to these assays as a indicator of cellular toxicity.
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Why is it important?
The ability to get as much information as possible from a single well greatly enhances the ability to follow small molecule inhibitors with the properties that are desired: specific and non-toxic.
Read the Original
This page is a summary of: Development of a Cell-Based High-Throughput Specificity Screen Using a Hepatitis C Virus-Bovine Viral Diarrhea Virus Dual Replicon Assay, Antimicrobial Agents and Chemotherapy, April 2005, ASM Journals,
DOI: 10.1128/aac.49.4.1346-1353.2005.
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