What is it about?
We developed a molecular diagnostic method that can detect almost all clinically available bla (β-lactamase) genes in various pathogenic isolates using only one unique multiplex PCR condition and sequencing of PCR products.
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Why is it important?
The large-scale bla detection ability of LARGE-SCALEblaFinder enables prompt application to detect almost all bla genes present in bacterial pathogens.
Perspectives
This method can be promptly used as an effective molecular diagnostic technique for the detection of bla genes in bacterial pathogens and will provide an important aid for monitoring the emergence and dissemination of bla genes and minimizing the spread of resistant bacteria.
Professor Sang Hee Lee
Myongji University
Read the Original
This page is a summary of: Fast and Accurate Large-Scale Detection of β-Lactamase Genes Conferring Antibiotic Resistance, Antimicrobial Agents and Chemotherapy, July 2015, ASM Journals,
DOI: 10.1128/aac.04634-14.
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