Consequences of producing DNA gyrase from a synthetic gyrBA operon in Salmonella enterica serovar Typhimurium

What is it about?

DNA gyrase is an essential enzyme in bacteria that is encoded by two genes located at separate places on the chromosome of Salmonella and many other bacteria. However, some bacteria have their two gyrase genes in an operon, where they are expressed as a single transcript. We have modified Salmonella to express its DNA gyrase from an operon and report the impact on the physiology and infectivity of this pathogenic bacterium.

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