What is it about?
Immune cells are responsible for antibody effector functions yet most assays measuring antibodies in serum use biochemical detection. We suggest that biological detection of antibodies or antibodies in immune complexes is possible by measuring the binding of cells to antibodies on a chip surface. The method works without labeling the cells since optical changes of the surface are monitored. We prove that different antibodies generate signals according to their inflamatory potential.
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Why is it important?
Biological readout of an antibody assay can generate data that are closer to in vivo antibody effects.
Perspectives
The technology could be used for fast label-free, point-of-care detection of serum antibodies if cells circulating in the blood of the tested individual can be used in the test.
József Prechl
Diagnosticum
Read the Original
This page is a summary of: Label-free detection of immune complexes with myeloid cells, Clinical & Experimental Immunology, May 2016, Wiley,
DOI: 10.1111/cei.12788.
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