What is it about?
CRISPR – short for clustered regularly interspaced short palindromic repeats – was discovered in prokaryotes, where it serves as an adaptive immune system that defends them against genetic invasion. It has been widely used in various applications, including in biomedical engineering. This review article summarizes the latest advancements in CRISPR technology for food-safety monitoring. Specifically, it introduces various CRISPR/Cas effector proteins and explains how each of them has been utilized in foodborne-pathogen detection. Additionally, it discusses such technologies’ current challenges and future opportunities.
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Why is it important?
Food safety is a major public health concern worldwide. Each year, hundreds of millions of people fall ill due to contaminated food, which causes hundreds of billions of dollars in economic loss. Therefore, there is an urgent need for rapid and accurate food-safety monitoring systems. CRISPR/Cas-based detection methods have the potential to solve many monitoring problems that conventional methods cannot. More importantly, as such technologies continue to develop, they may become capable of addressing a wide range of global socio-economic problems that arise from poor food-safety practices.
Perspectives
This review summarizes fundamental knowledge of CRISPR/Cas-based detection systems and their state-of-the-art development. It also discusses the challenges and limitations of the current technology, as well as how it might usefully be applied in other areas of the food industry. Such discussions include detailed descriptions of existing problems and their potential solutions. This will enable readers from various fields to learn about potential future directions of research in this area, and might even inspire their future work.
Jiyong Shin
University of Illinois at Urbana-Champaign
Read the Original
This page is a summary of: Recent advances in CRISPR‐based systems for the detection of foodborne pathogens, Comprehensive Reviews in Food Science and Food Safety, April 2022, Wiley,
DOI: 10.1111/1541-4337.12956.
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