What is it about?
It addresses the application of synthetic small RNA (sRNA) system in Methylorubrum extorquens, which is an important one carbon bioeconomic bacteria but limited by genetic manipulation. In this work, we found the native Hfq in M. extorquens strains worked well with E. coli MicC and had better efficiency than E. coli Hfq. Interference of target crtI gene using this synthetic sRNA system in M. extorquens AM1 reduce 86% of carotenoid production, which provided a genetic tool for manipulation of M. extorquens.
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Why is it important?
As a newly established genetic tool for gene repression, synthetic sRNA system based on E.coli sourced MicC and chaperon Hfq has already used in some strains. However, the native Hfq had recognition issues with E. coli MicC though it is widespread in bacteria, and the E. coli Hfq is simultaneously introduce to make the system work.
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This page is a summary of: Gene repression using synthetic small regulatory RNA in
Methylorubrum extorquens, Journal of Applied Microbiology, June 2021, Wiley,
DOI: 10.1111/jam.15159.
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