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This page is a summary of: Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER, Acta Crystallographica Section D Structural Biology, July 2020, International Union of Crystallography,
DOI: 10.1107/s2059798320008049.
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This resource exemplifies the use of ARCIMBOLDO_SHREDDER on data from the micro electron diffraction technique. Both SHREDDER sequential and SHREDDER spheres will be using the dataset deposited under the PDB id 6V8R, corresponding to 1.6 Å microED data from the proteinase K.
Presentation at the ECM32 in Vienna about ARCIMBOLDO's application to MicroED data
Here, we present success in structure determination of amyloidogenic peptides by MicroED using both molecular replacement and direct methods phasing. We also present the use of ARCIMBOLDO SHREDDER, a fragment-based MR method, coupled with standard autobuilding software to determine the atomic structures from Proteinase K (ProK) data collected by MicroED.
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